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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leuconostoc mesenteroides subsp. mesenteroides UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP311944LFG
CSB-EP311944LFG
CSB-BP311944LFG
CSB-MP311944LFG
CSB-EP311944LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP311945LFG
CSB-EP311945LFG
CSB-BP311945LFG
CSB-MP311945LFG
CSB-EP311945LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L35 (rpmI) CSB-YP311946LFG
CSB-EP311946LFG
CSB-BP311946LFG
CSB-MP311946LFG
CSB-EP311946LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Chaperone protein DnaK (dnaK), partial CSB-YP311947LFG
CSB-EP311947LFG
CSB-BP311947LFG
CSB-MP311947LFG
CSB-EP311947LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Cytidylate kinase (cmk) CSB-YP311948LFG
CSB-EP311948LFG
CSB-BP311948LFG
CSB-MP311948LFG
CSB-EP311948LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP311949LFG
CSB-EP311949LFG
CSB-BP311949LFG
CSB-MP311949LFG
CSB-EP311949LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP311950LFG
CSB-EP311950LFG
CSB-BP311950LFG
CSB-MP311950LFG
CSB-EP311950LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides DNA repair protein recO (recO) CSB-YP311951LFG
CSB-EP311951LFG
CSB-BP311951LFG
CSB-MP311951LFG
CSB-EP311951LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP311952LFG
CSB-EP311952LFG
CSB-BP311952LFG
CSB-MP311952LFG
CSB-EP311952LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides N-acetyldiaminopimelate deacetylase (LEUM_0669) CSB-YP311953LFG
CSB-EP311953LFG
CSB-BP311953LFG
CSB-MP311953LFG
CSB-EP311953LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides UPF0398 protein LEUM_0652 (LEUM_0652) CSB-YP311954LFG
CSB-EP311954LFG
CSB-BP311954LFG
CSB-MP311954LFG
CSB-EP311954LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Protease HtpX homolog (htpX), partial CSB-YP311955LFG1
CSB-EP311955LFG1
CSB-BP311955LFG1
CSB-MP311955LFG1
CSB-EP311955LFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP311956LFG
CSB-EP311956LFG
CSB-BP311956LFG
CSB-MP311956LFG
CSB-EP311956LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Hydroxyethylthiazole kinase 1 (thiM1) CSB-YP311957LFG
CSB-EP311957LFG
CSB-BP311957LFG
CSB-MP311957LFG
CSB-EP311957LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Methionine import ATP-binding protein MetN (metN) CSB-YP311958LFG
CSB-EP311958LFG
CSB-BP311958LFG
CSB-MP311958LFG
CSB-EP311958LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L13 (rplM) CSB-YP311959LFG
CSB-EP311959LFG
CSB-BP311959LFG
CSB-MP311959LFG
CSB-EP311959LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L17 (rplQ) CSB-YP311960LFG
CSB-EP311960LFG
CSB-BP311960LFG
CSB-MP311960LFG
CSB-EP311960LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 30S ribosomal protein S17 (rpsQ) CSB-YP311961LFG
CSB-EP311961LFG
CSB-BP311961LFG
CSB-MP311961LFG
CSB-EP311961LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L2 (rplB) CSB-YP311962LFG
CSB-EP311962LFG
CSB-BP311962LFG
CSB-MP311962LFG
CSB-EP311962LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides 50S ribosomal protein L3 (rplC) CSB-YP311963LFG
CSB-EP311963LFG
CSB-BP311963LFG
CSB-MP311963LFG
CSB-EP311963LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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