Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Protein inturned (Intu), partial CSB-YP312308MO
CSB-EP312308MO
CSB-BP312308MO
CSB-MP312308MO
CSB-EP312308MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein FAM210A (FAM210A), partial CSB-YP312309BO1
CSB-EP312309BO1
CSB-BP312309BO1
CSB-MP312309BO1
CSB-EP312309BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 30S ribosomal protein S7 (rpsG) CSB-YP312310CEG
CSB-EP312310CEG
CSB-BP312310CEG
CSB-MP312310CEG
CSB-EP312310CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 50S ribosomal protein L2 (rplB) CSB-YP312311CEG
CSB-EP312311CEG
CSB-BP312311CEG
CSB-MP312311CEG
CSB-EP312311CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 30S ribosomal protein S11 (rpsK) CSB-YP312312CEG
CSB-EP312312CEG
CSB-BP312312CEG
CSB-MP312312CEG
CSB-EP312312CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 50S ribosomal protein L20 (rplT) CSB-YP312313CEG
CSB-EP312313CEG
CSB-BP312313CEG
CSB-MP312313CEG
CSB-EP312313CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii 60 kDa chaperonin (groL), partial CSB-YP312314CEG
CSB-EP312314CEG
CSB-BP312314CEG
CSB-MP312314CEG
CSB-EP312314CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carsonella ruddii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) CSB-YP312315CEG
CSB-EP312315CEG
CSB-BP312315CEG
CSB-MP312315CEG
CSB-EP312315CEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein SlyX homolog (slyX) CSB-YP312316XBB
CSB-EP312316XBB
CSB-BP312316XBB
CSB-MP312316XBB
CSB-EP312316XBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lambda-carrageenase (cglA), partial CSB-YP312317PTS
CSB-EP312317PTS
CSB-BP312317PTS
CSB-MP312317PTS
CSB-EP312317PTS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas chlororaphis Copper-containing nitrite reductase (nirK) CSB-YP312318PUG
CSB-EP312318PUG
CSB-BP312318PUG
CSB-MP312318PUG
CSB-EP312318PUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arachis hypogaea Endochitinase 4 CSB-YP312319ANE
CSB-EP312319ANE
CSB-BP312319ANE
CSB-MP312319ANE
CSB-EP312319ANE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Betula papyrifera Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP312320BGN
CSB-EP312320BGN
CSB-BP312320BGN
CSB-MP312320BGN
CSB-EP312320BGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chromosome stability protein 9 (CST9) CSB-YP312321SVG
CSB-EP312321SVG
CSB-BP312321SVG
CSB-MP312321SVG
CSB-EP312321SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Seipin FLD1 (FLD1), partial CSB-YP312322SVG1
CSB-EP312322SVG1
CSB-BP312322SVG1
CSB-MP312322SVG1
CSB-EP312322SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Mitogen-activated protein kinase homolog D5 CSB-YP312323EWE
CSB-EP312323EWE
CSB-BP312323EWE
CSB-MP312323EWE
CSB-EP312323EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Signal transduction histidine-protein kinase AtoS (atoS), partial CSB-YP312324ENV1
CSB-EP312324ENV1
CSB-BP312324ENV1
CSB-MP312324ENV1
CSB-EP312324ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Biogenesis of lysosome-related organelles complex 1 subunit BLS1 (BLS1) CSB-YP312325SVG
CSB-EP312325SVG
CSB-BP312325SVG
CSB-MP312325SVG
CSB-EP312325SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Tyrosine decarboxylase 2 (TYRDC-2), partial CSB-YP312326PGL
CSB-EP312326PGL
CSB-BP312326PGL
CSB-MP312326PGL
CSB-EP312326PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida maltosa S- (hydroxymethyl)glutathione dehydrogenase CSB-YP312327CZJ
CSB-EP312327CZJ
CSB-BP312327CZJ
CSB-MP312327CZJ
CSB-EP312327CZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>