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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces coelicolor Actinorhodin polyketide putative beta-ketoacyl synthase 2 (SCO5088) CSB-YP312513FOB
CSB-EP312513FOB
CSB-BP312513FOB
CSB-MP312513FOB
CSB-EP312513FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Persea americana Polygalacturonase CSB-YP312514PGI
CSB-EP312514PGI
CSB-BP312514PGI
CSB-MP312514PGI
CSB-EP312514PGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N-acetylmuramoyl-L-alanine amidase LytC (lytC) CSB-YP312516BRJ
CSB-EP312516BRJ
CSB-BP312516BRJ
CSB-MP312516BRJ
CSB-EP312516BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP312517LNG
CSB-EP312517LNG
CSB-BP312517LNG
CSB-MP312517LNG
CSB-EP312517LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Acetolactate synthase large subunit (ilvB), partial CSB-YP312518LNG
CSB-EP312518LNG
CSB-BP312518LNG
CSB-MP312518LNG
CSB-EP312518LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis L-threonine dehydratase biosynthetic IlvA (ilvA) CSB-YP312519LNG
CSB-EP312519LNG
CSB-BP312519LNG
CSB-MP312519LNG
CSB-EP312519LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine synthetase 2 (glnII) CSB-YP312520RKO
CSB-EP312520RKO
CSB-BP312520RKO
CSB-MP312520RKO
CSB-EP312520RKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. C-phycoerythrin class 1 subunit alpha (cpeA) CSB-YP312521SSK
CSB-EP312521SSK
CSB-BP312521SSK
CSB-MP312521SSK
CSB-EP312521SSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein ECM9 (ECM9) CSB-YP312523SVG
CSB-EP312523SVG
CSB-BP312523SVG
CSB-MP312523SVG
CSB-EP312523SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana sylvestris Trypsin inhibitor 1 CSB-YP312524NHD
CSB-EP312524NHD
CSB-BP312524NHD
CSB-MP312524NHD
CSB-EP312524NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax mediterranei Gas vesicle protein gvpJ (gvpJ) CSB-YP312525HTS
CSB-EP312525HTS
CSB-BP312525HTS
CSB-MP312525HTS
CSB-EP312525HTS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Wound-induced protein (WIN) CSB-YP312526GGV
CSB-EP312526GGV
CSB-BP312526GGV
CSB-MP312526GGV
CSB-EP312526GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP312527HMI
CSB-EP312527HMI
CSB-BP312527HMI
CSB-MP312527HMI
CSB-EP312527HMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E5 (E5) CSB-YP312528HMI
CSB-EP312528HMI
CSB-BP312528HMI
CSB-MP312528HMI
CSB-EP312528HMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygmy chimpanzee papillomavirus type 1 Major capsid protein L1 (L1), partial CSB-YP312529PXM
CSB-EP312529PXM
CSB-BP312529PXM
CSB-MP312529PXM
CSB-EP312529PXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Potassium voltage-gated channel protein eag (eag), partial CSB-YP312530DLU
CSB-EP312530DLU
CSB-BP312530DLU
CSB-MP312530DLU
CSB-EP312530DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Recombination protein RecR (recR) CSB-YP312531SIU
CSB-EP312531SIU
CSB-BP312531SIU
CSB-MP312531SIU
CSB-EP312531SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus sylvestris Dihydropinosylvin synthase CSB-YP312532PYS
CSB-EP312532PYS
CSB-BP312532PYS
CSB-MP312532PYS
CSB-EP312532PYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-galactose translocator 1 (ugtp-1), partial CSB-YP312533CXY1
CSB-EP312533CXY1
CSB-BP312533CXY1
CSB-MP312533CXY1
CSB-EP312533CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U3 small nucleolar RNA-associated protein 6 (UTP6) CSB-YP312534SVG
CSB-EP312534SVG
CSB-BP312534SVG
CSB-MP312534SVG
CSB-EP312534SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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