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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Southampton virus Genome polyprotein (ORF1), partial CSB-YP312005SIX
CSB-EP312005SIX
CSB-BP312005SIX
CSB-MP312005SIX
CSB-EP312005SIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Actin-like protein ARP10 (ARP10) CSB-YP312006SVG
CSB-EP312006SVG
CSB-BP312006SVG
CSB-MP312006SVG
CSB-EP312006SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 4 (GP4), partial CSB-YP312007PQC1
CSB-EP312007PQC1
CSB-BP312007PQC1
CSB-MP312007PQC1
CSB-EP312007PQC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shallot virus X Movement and silencing protein TGBp1 (ORF2) CSB-YP312008SFT
CSB-EP312008SFT
CSB-BP312008SFT
CSB-MP312008SFT
CSB-EP312008SFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 54S ribosomal protein L1, mitochondrial (MRPL1) CSB-YP312009SVG
CSB-EP312009SVG
CSB-BP312009SVG
CSB-MP312009SVG
CSB-EP312009SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri 10 kDa chaperonin (groS) CSB-YP312010LAU
CSB-EP312010LAU
CSB-BP312010LAU
CSB-MP312010LAU
CSB-EP312010LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri Cysteine--tRNA ligase (cysS) CSB-YP312011LAU
CSB-EP312011LAU
CSB-BP312011LAU
CSB-MP312011LAU
CSB-EP312011LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA polymerase epsilon subunit D (DPB4) CSB-YP312012SVG
CSB-EP312012SVG
CSB-BP312012SVG
CSB-MP312012SVG
CSB-EP312012SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP synthase protein MI25 (AtMg00640), partial CSB-YP312013DOA1
CSB-EP312013DOA1
CSB-BP312013DOA1
CSB-MP312013DOA1
CSB-EP312013DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Pro-opiomelanocortin A (pomca) CSB-YP312014OEI
CSB-EP312014OEI
CSB-BP312014OEI
CSB-MP312014OEI
CSB-EP312014OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Palmitoyltransferase SWF1 (SWF1), partial CSB-YP312015SVG1
CSB-EP312015SVG1
CSB-BP312015SVG1
CSB-MP312015SVG1
CSB-EP312015SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 1-alpha CSB-YP312016TLT
CSB-EP312016TLT
CSB-BP312016TLT
CSB-MP312016TLT
CSB-EP312016TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mxiC (mxiC) CSB-YP312017SZB
CSB-EP312017SZB
CSB-BP312017SZB
CSB-MP312017SZB
CSB-EP312017SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chromosome segregation in meiosis protein 3 (CSM3) CSB-YP312018SVG
CSB-EP312018SVG
CSB-BP312018SVG
CSB-MP312018SVG
CSB-EP312018SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Suppressor of stem-loop protein 1 (SSL1) CSB-YP312019SVG
CSB-EP312019SVG
CSB-BP312019SVG
CSB-MP312019SVG
CSB-EP312019SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 17 Non-structural protein P8 (S10) CSB-YP312020BHU
CSB-EP312020BHU
CSB-BP312020BHU
CSB-MP312020BHU
CSB-EP312020BHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP312021LAU
CSB-EP312021LAU
CSB-BP312021LAU
CSB-MP312021LAU
CSB-EP312021LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri 50S ribosomal protein L4 (rplD) CSB-YP312022LAU
CSB-EP312022LAU
CSB-BP312022LAU
CSB-MP312022LAU
CSB-EP312022LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri D-alanine--D-alanine ligase (ddl) CSB-YP312023LAU
CSB-EP312023LAU
CSB-BP312023LAU
CSB-MP312023LAU
CSB-EP312023LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine coronavirus Non-structural protein 7b (7b) CSB-YP312024CCP
CSB-EP312024CCP
CSB-BP312024CCP
CSB-MP312024CCP
CSB-EP312024CCP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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