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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus oryzae Alpha-amylase A type-1/2 (amy1) CSB-YP313837DPA
CSB-EP313837DPA
CSB-BP313837DPA
CSB-MP313837DPA
CSB-EP313837DPA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Putative L-ribulose-5-phosphate 4-epimerase UlaF (ulaF) CSB-YP313838SGE
CSB-EP313838SGE
CSB-BP313838SGE
CSB-MP313838SGE
CSB-EP313838SGE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus austini Gamma-conotoxin-like As7a CSB-YP313839COA
CSB-EP313839COA
CSB-BP313839COA
CSB-MP313839COA
CSB-EP313839COA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein PS1 (csp1), partial CSB-YP313840CQK
CSB-EP313840CQK
CSB-BP313840CQK
CSB-MP313840CQK
CSB-EP313840CQK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glutamate 5-kinase (proB) CSB-YP313841DWY
CSB-EP313841DWY
CSB-BP313841DWY
CSB-MP313841DWY
CSB-EP313841DWY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Toxin APE 2-2 CSB-YP313842DNA
CSB-EP313842DNA
CSB-BP313842DNA
CSB-MP313842DNA
CSB-EP313842DNA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus acidophilus Deoxyadenosine kinase (LBA1949) CSB-YP313843LME
CSB-EP313843LME
CSB-BP313843LME
CSB-MP313843LME
CSB-EP313843LME-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposons Tn1721 resolvase (tnpR) CSB-YP313844ENL
CSB-EP313844ENL
CSB-BP313844ENL
CSB-MP313844ENL
CSB-EP313844ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinia equina Equinatoxin-1 CSB-YP313845ACN
CSB-EP313845ACN
CSB-BP313845ACN
CSB-MP313845ACN
CSB-EP313845ACN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9) CSB-YP313846RDE
CSB-EP313846RDE
CSB-BP313846RDE
CSB-MP313846RDE
CSB-EP313846RDE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group O Protein Tat CSB-YP313847HLT
CSB-EP313847HLT
CSB-BP313847HLT
CSB-MP313847HLT
CSB-EP313847HLT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase for insertion sequence-like element IS431mec (tnp) CSB-YP313848FLF
CSB-EP313848FLF
CSB-BP313848FLF
CSB-MP313848FLF
CSB-EP313848FLF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Rev (rev) CSB-YP313849HLP
CSB-EP313849HLP
CSB-BP313849HLP
CSB-MP313849HLP
CSB-EP313849HLP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa magnifica Vespid chemotactic peptide 5e CSB-YP313850VBQ
CSB-EP313850VBQ
CSB-BP313850VBQ
CSB-MP313850VBQ
CSB-EP313850VBQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polistes dominula Dominulin-B CSB-YP313851POG
CSB-EP313851POG
CSB-BP313851POG
CSB-MP313851POG
CSB-EP313851POG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Conotoxin Tx3.5-a CSB-YP313852DWJ
CSB-EP313852DWJ
CSB-BP313852DWJ
CSB-MP313852DWJ
CSB-EP313852DWJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid II:glycine glycyltransferase (femX) CSB-YP313853FKZ
CSB-EP313853FKZ
CSB-BP313853FKZ
CSB-MP313853FKZ
CSB-EP313853FKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protonectarina sylveirae Mastoparan CSB-YP313854PSS
CSB-EP313854PSS
CSB-BP313854PSS
CSB-MP313854PSS
CSB-EP313854PSS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP313855FKZ
CSB-EP313855FKZ
CSB-BP313855FKZ
CSB-MP313855FKZ
CSB-EP313855FKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-regulated surface determinant protein A (isdA) CSB-YP313856FKZ
CSB-EP313856FKZ
CSB-BP313856FKZ
CSB-MP313856FKZ
CSB-EP313856FKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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