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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida maltosa Oleate-induced peroxisomal protein POX18 (POX18) CSB-YP312410CZJ
CSB-EP312410CZJ
CSB-BP312410CZJ
CSB-MP312410CZJ
CSB-EP312410CZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrus tristeza virus Capsid protein CSB-YP312411CKY
CSB-EP312411CKY
CSB-BP312411CKY
CSB-MP312411CKY
CSB-EP312411CKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Large structural phosphoprotein (U11), partial CSB-YP312412HJZ
CSB-BP312412HJZ
CSB-MP312412HJZ
CSB-EP312412HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Guanine nucleotide-binding protein subunit alpha (fadA) CSB-YP312413EKF
CSB-EP312413EKF
CSB-BP312413EKF
CSB-MP312413EKF
CSB-EP312413EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multiple sugar-binding transport system permease protein msmF (msmF), partial CSB-YP312414FMR1
CSB-EP312414FMR1
CSB-BP312414FMR1
CSB-MP312414FMR1
CSB-EP312414FMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus tremuloides Caffeic acid 3-O-methyltransferase 1 (OMT1) CSB-YP312415EXR
CSB-EP312415EXR
CSB-BP312415EXR
CSB-MP312415EXR
CSB-EP312415EXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP312416FNU
CSB-EP312416FNU
CSB-BP312416FNU
CSB-MP312416FNU
CSB-EP312416FNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Resistance to glucose repression protein 1 (REG1), partial CSB-YP312417SVG
CSB-EP312417SVG
CSB-BP312417SVG
CSB-MP312417SVG
CSB-EP312417SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Chlorophyll a-b binding protein 48, chloroplastic (CAB48), partial CSB-YP312418ZAX1
CSB-EP312418ZAX1
CSB-BP312418ZAX1
CSB-MP312418ZAX1
CSB-EP312418ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichodiene synthase (TRI5) CSB-YP312419FDN
CSB-EP312419FDN
CSB-BP312419FDN
CSB-MP312419FDN
CSB-EP312419FDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS1106 CSB-YP312420NBK
CSB-EP312420NBK
CSB-BP312420NBK
CSB-MP312420NBK
CSB-EP312420NBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice stripe virus Movement protein (pc4) CSB-YP312421REQ
CSB-EP312421REQ
CSB-BP312421REQ
CSB-MP312421REQ
CSB-EP312421REQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus thunbergii Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP312423EVZ
CSB-EP312423EVZ
CSB-BP312423EVZ
CSB-MP312423EVZ
CSB-EP312423EVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litopenaeus vannamei Chymotrypsin BI CSB-YP312424LHD
CSB-EP312424LHD
CSB-BP312424LHD
CSB-MP312424LHD
CSB-EP312424LHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant White bream virus Replicase polyprotein 1a (1a), partial CSB-YP312425WAJ
CSB-EP312425WAJ
CSB-BP312425WAJ
CSB-MP312425WAJ
CSB-EP312425WAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Hydantoin racemase (hyuE) CSB-YP312426PVR
CSB-EP312426PVR
CSB-BP312426PVR
CSB-MP312426PVR
CSB-EP312426PVR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Capsid protein (ORF IV) CSB-YP312427CFD
CSB-EP312427CFD
CSB-BP312427CFD
CSB-MP312427CFD
CSB-EP312427CFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Enzymatic polyprotein (ORF V), partial CSB-YP312428CFD
CSB-EP312428CFD
CSB-BP312428CFD
CSB-MP312428CFD
CSB-EP312428CFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis UDP-N-acetylglucosamine transporter YEA4 (YEA4), partial CSB-YP312429KBK
CSB-EP312429KBK
CSB-BP312429KBK
CSB-MP312429KBK
CSB-EP312429KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Ent-cassa-12,15-diene synthase (KSL7), partial CSB-YP312430OFF
CSB-EP312430OFF
CSB-BP312430OFF
CSB-MP312430OFF
CSB-EP312430OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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