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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin hydrogenase (hmd) CSB-YP312535MSI
CSB-EP312535MSI
CSB-BP312535MSI
CSB-MP312535MSI
CSB-EP312535MSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus D-alanine--D-alanine ligase (ddl) CSB-YP312536SIU
CSB-EP312536SIU
CSB-BP312536SIU
CSB-MP312536SIU
CSB-EP312536SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 8.1 kDa protein in Gp30-rIII intergenic region (y12I) CSB-YP312537EDZ
CSB-EP312537EDZ
CSB-BP312537EDZ
CSB-MP312537EDZ
CSB-EP312537EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupiennius salei Protein krueppel (Kr) CSB-YP312538CUR
CSB-EP312538CUR
CSB-BP312538CUR
CSB-MP312538CUR
CSB-EP312538CUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Chlorophyllide reductase 35.5 kDa chain (bchX) CSB-YP312539RLF
CSB-EP312539RLF
CSB-BP312539RLF
CSB-MP312539RLF
CSB-EP312539RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP312540FCT
CSB-EP312540FCT
CSB-BP312540FCT
CSB-MP312540FCT
CSB-EP312540FCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein TBF1 (TBF1), partial CSB-YP312541SVG
CSB-EP312541SVG
CSB-BP312541SVG
CSB-MP312541SVG
CSB-EP312541SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella frigidimarina Uncharacterized protein Sfri_3691 (Sfri_3691) CSB-YP312542SYT
CSB-EP312542SYT
CSB-BP312542SYT
CSB-MP312542SYT
CSB-EP312542SYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mucin-1 (Muc1), partial CSB-YP312543MO
CSB-EP312543MO
CSB-BP312543MO
CSB-MP312543MO
CSB-EP312543MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP312544HNC
CSB-EP312544HNC
CSB-BP312544HNC
CSB-MP312544HNC
CSB-EP312544HNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Mating-type pheromone BAP1 (2), partial CSB-YP312545SXR
CSB-EP312545SXR
CSB-BP312545SXR
CSB-MP312545SXR
CSB-EP312545SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nucleoporin NUP100/NSP100 (NUP100), partial CSB-YP312546SVG
CSB-EP312546SVG
CSB-BP312546SVG
CSB-MP312546SVG
CSB-EP312546SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA (uracil-O (2)-)-methyltransferase, partial CSB-YP312547SVG
CSB-EP312547SVG
CSB-BP312547SVG
CSB-MP312547SVG
CSB-EP312547SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YPL025C (YPL025C) CSB-YP312548SVG
CSB-EP312548SVG
CSB-BP312548SVG
CSB-MP312548SVG
CSB-EP312548SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein Q300 (Hpvc2), partial CSB-YP312549MO1
CSB-EP312549MO1
CSB-BP312549MO1
CSB-MP312549MO1
CSB-EP312549MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar transporter chaperone 3 (VTC3), partial CSB-YP312550SVG
CSB-EP312550SVG
CSB-BP312550SVG
CSB-MP312550SVG
CSB-EP312550SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidic phosphoprotein (PCEMA1) CSB-YP312551EWO
CSB-EP312551EWO
CSB-BP312551EWO
CSB-MP312551EWO
CSB-EP312551EWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum ATP synthase delta chain, chloroplastic (ATPD) CSB-YP312552EWE
CSB-EP312552EWE
CSB-BP312552EWE
CSB-MP312552EWE
CSB-EP312552EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribonuclease P protein component, mitochondrial (RPM2), partial CSB-YP312553SVG
CSB-EP312553SVG
CSB-BP312553SVG
CSB-MP312553SVG
CSB-EP312553SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Long chronological lifespan protein 1 (LCL1) CSB-YP312554SVG
CSB-EP312554SVG
CSB-BP312554SVG
CSB-MP312554SVG
CSB-EP312554SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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