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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactococcus lactis subsp. cremoris UPF0176 protein LACR_1324 (LACR_1324) CSB-YP312657LBE
CSB-EP312657LBE
CSB-BP312657LBE
CSB-MP312657LBE
CSB-EP312657LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP312658LBE
CSB-EP312658LBE
CSB-BP312658LBE
CSB-MP312658LBE
CSB-EP312658LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 50S ribosomal protein L27 (rpmA) CSB-YP312659LBE
CSB-EP312659LBE
CSB-BP312659LBE
CSB-MP312659LBE
CSB-EP312659LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Putative sporulation transcription regulator WhiA (whiA) CSB-YP312660LBE
CSB-EP312660LBE
CSB-BP312660LBE
CSB-MP312660LBE
CSB-EP312660LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 50S ribosomal protein L19 (rplS) CSB-YP312661LBE
CSB-EP312661LBE
CSB-BP312661LBE
CSB-MP312661LBE
CSB-EP312661LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Hydrogenase expression/formation protein hupH (hupH) CSB-YP312662RLC
CSB-EP312662RLC
CSB-BP312662RLC
CSB-MP312662RLC
CSB-EP312662RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GLC7-interacting protein 3 (GIP3), partial CSB-YP312663SVG
CSB-EP312663SVG
CSB-BP312663SVG
CSB-MP312663SVG
CSB-EP312663SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Serralysin (aprA), partial CSB-YP312664EZX1
CSB-BP312664EZX1
CSB-MP312664EZX1
CSB-EP312664EZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Oxaloacetate decarboxylase gamma chain 2 (oadG2), partial CSB-YP312665SXB1
CSB-EP312665SXB1
CSB-BP312665SXB1
CSB-MP312665SXB1
CSB-EP312665SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Seripauperin-10 (PAU10) CSB-YP312666SVG
CSB-EP312666SVG
CSB-BP312666SVG
CSB-MP312666SVG
CSB-EP312666SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Elongin-C (ELC1) CSB-YP312667SVG
CSB-EP312667SVG
CSB-BP312667SVG
CSB-MP312667SVG
CSB-EP312667SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional regulator MET31 (MET31) CSB-YP312668SVG
CSB-EP312668SVG
CSB-BP312668SVG
CSB-MP312668SVG
CSB-EP312668SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Transcriptional repressor NrdR (nrdR) CSB-YP312669LBE
CSB-EP312669LBE
CSB-BP312669LBE
CSB-MP312669LBE
CSB-EP312669LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Circumsporozoite protein (CS) CSB-YP312670PMQ
CSB-EP312670PMQ
CSB-BP312670PMQ
CSB-MP312670PMQ
CSB-EP312670PMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Erythronolide synthase, modules 5 and 6 (eryA), partial CSB-YP312671SAL
CSB-EP312671SAL
CSB-BP312671SAL
CSB-MP312671SAL
CSB-EP312671SAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase synthesis protein 25, mitochondrial (ATP25), partial CSB-YP312672SVG
CSB-EP312672SVG
CSB-BP312672SVG
CSB-MP312672SVG
CSB-EP312672SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell wall mannoprotein PIR1 (PIR1) CSB-YP312673SVG
CSB-EP312673SVG
CSB-BP312673SVG
CSB-MP312673SVG
CSB-EP312673SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Plasma membrane ATPase 4 (PMA4), partial CSB-YP312674NHC
CSB-EP312674NHC
CSB-BP312674NHC
CSB-MP312674NHC
CSB-EP312674NHC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Proteinase inhibitor I-A (TIMPB) CSB-YP312675NHE
CSB-EP312675NHE
CSB-BP312675NHE
CSB-MP312675NHE
CSB-EP312675NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Trigger factor (tig) CSB-YP312676LBE
CSB-EP312676LBE
CSB-BP312676LBE
CSB-MP312676LBE
CSB-EP312676LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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