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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP312575PUC
CSB-EP312575PUC
CSB-BP312575PUC
CSB-MP312575PUC
CSB-EP312575PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA sulfurtransferase (thiI) CSB-YP312576PUC
CSB-EP312576PUC
CSB-BP312576PUC
CSB-MP312576PUC
CSB-EP312576PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP312577PUC
CSB-EP312577PUC
CSB-BP312577PUC
CSB-MP312577PUC
CSB-EP312577PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP312578PUC
CSB-EP312578PUC
CSB-BP312578PUC
CSB-MP312578PUC
CSB-EP312578PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP312579PUC
CSB-EP312579PUC
CSB-BP312579PUC
CSB-MP312579PUC
CSB-EP312579PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Adenylosuccinate synthetase (purA) CSB-YP312580PUC
CSB-EP312580PUC
CSB-BP312580PUC
CSB-MP312580PUC
CSB-EP312580PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urease accessory protein UreD (ureD) CSB-YP312581PUC
CSB-EP312581PUC
CSB-BP312581PUC
CSB-MP312581PUC
CSB-EP312581PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein grpE (grpE) CSB-YP312582PUC
CSB-EP312582PUC
CSB-BP312582PUC
CSB-MP312582PUC
CSB-EP312582PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA pseudouridine synthase B (truB) CSB-YP312583PUC
CSB-EP312583PUC
CSB-BP312583PUC
CSB-MP312583PUC
CSB-EP312583PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L25 (rplY) CSB-YP312584PUC
CSB-EP312584PUC
CSB-BP312584PUC
CSB-MP312584PUC
CSB-EP312584PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Uracil phosphoribosyltransferase (upp) CSB-YP312585PUC
CSB-EP312585PUC
CSB-BP312585PUC
CSB-MP312585PUC
CSB-EP312585PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP312586PUC
CSB-EP312586PUC
CSB-BP312586PUC
CSB-MP312586PUC
CSB-EP312586PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP312587PUC
CSB-EP312587PUC
CSB-BP312587PUC
CSB-MP312587PUC
CSB-EP312587PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein MraZ (mraZ) CSB-YP312588PUC
CSB-EP312588PUC
CSB-BP312588PUC
CSB-MP312588PUC
CSB-EP312588PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0234 protein PA14_57130 (PA14_57130) CSB-YP312589PUC
CSB-EP312589PUC
CSB-BP312589PUC
CSB-MP312589PUC
CSB-EP312589PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Chromosome initiation inhibitor (iciA) CSB-YP312590PUC
CSB-EP312590PUC
CSB-BP312590PUC
CSB-MP312590PUC
CSB-EP312590PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA repair protein recO (recO) CSB-YP312591PUC
CSB-EP312591PUC
CSB-BP312591PUC
CSB-MP312591PUC
CSB-EP312591PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein CysZ homolog (cysZ), partial CSB-YP312592PUC1
CSB-EP312592PUC1
CSB-BP312592PUC1
CSB-MP312592PUC1
CSB-EP312592PUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein SlyX homolog (slyX) CSB-YP312593PUC
CSB-EP312593PUC
CSB-BP312593PUC
CSB-MP312593PUC
CSB-EP312593PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP312594PUC
CSB-EP312594PUC
CSB-BP312594PUC
CSB-MP312594PUC
CSB-EP312594PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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