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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermoplasma acidophilum Bifunctional protein FolD (folD) CSB-YP313143TND
CSB-EP313143TND
CSB-BP313143TND
CSB-MP313143TND
CSB-EP313143TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Major head protein Gp17 (17) CSB-YP313144MLC
CSB-EP313144MLC
CSB-BP313144MLC
CSB-MP313144MLC
CSB-EP313144MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 25 protein (25) CSB-YP313145MLC
CSB-EP313145MLC
CSB-BP313145MLC
CSB-MP313145MLC
CSB-EP313145MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phycobilisome 32.3 kDa linker polypeptide, phycocyanin-associated, rod (cpcC) CSB-YP313146FQA
CSB-EP313146FQA
CSB-BP313146FQA
CSB-MP313146FQA
CSB-EP313146FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 37 protein (37) CSB-YP313147MLC
CSB-EP313147MLC
CSB-BP313147MLC
CSB-MP313147MLC
CSB-EP313147MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 39 protein (39) CSB-YP313148MLC
CSB-EP313148MLC
CSB-BP313148MLC
CSB-MP313148MLC
CSB-EP313148MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 45 protein (45) CSB-YP313149MLC
CSB-EP313149MLC
CSB-BP313149MLC
CSB-MP313149MLC
CSB-EP313149MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 54 protein (54) CSB-YP313150MLC
CSB-EP313150MLC
CSB-BP313150MLC
CSB-MP313150MLC
CSB-EP313150MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 52 protein (52) CSB-YP313151MLC
CSB-EP313151MLC
CSB-BP313151MLC
CSB-MP313151MLC
CSB-EP313151MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 60 protein (60) CSB-YP313152MLC
CSB-EP313152MLC
CSB-BP313152MLC
CSB-MP313152MLC
CSB-EP313152MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Minor tail protein Gp6 (6) CSB-YP313153MLC
CSB-EP313153MLC
CSB-BP313153MLC
CSB-MP313153MLC
CSB-EP313153MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 65 protein (65) CSB-YP313154MLC
CSB-EP313154MLC
CSB-BP313154MLC
CSB-MP313154MLC
CSB-EP313154MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 72 protein (72) CSB-YP313155MLC
CSB-EP313155MLC
CSB-BP313155MLC
CSB-MP313155MLC
CSB-EP313155MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 80 protein (80) CSB-YP313156MLC
CSB-EP313156MLC
CSB-BP313156MLC
CSB-MP313156MLC
CSB-EP313156MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Homoserine O-acetyltransferase (metX) CSB-YP313157LET
CSB-EP313157LET
CSB-BP313157LET
CSB-MP313157LET
CSB-EP313157LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis CinA-like protein (LBL_1257) CSB-YP313158LET
CSB-EP313158LET
CSB-BP313158LET
CSB-MP313158LET
CSB-EP313158LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L9 (rplI) CSB-YP313159LET
CSB-EP313159LET
CSB-BP313159LET
CSB-MP313159LET
CSB-EP313159LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Trigger factor (tig) CSB-YP313160LET
CSB-EP313160LET
CSB-BP313160LET
CSB-MP313160LET
CSB-EP313160LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 60 kDa chaperonin (groL), partial CSB-YP313161LET
CSB-EP313161LET
CSB-BP313161LET
CSB-MP313161LET
CSB-EP313161LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium phage L5 Gene 85 protein (85) CSB-YP313162MLC
CSB-EP313162MLC
CSB-BP313162MLC
CSB-MP313162MLC
CSB-EP313162MLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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