Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP313369EOD
CSB-EP313369EOD
CSB-BP313369EOD
CSB-MP313369EOD
CSB-EP313369EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Penicillin-insensitive murein endopeptidase (mepA) CSB-YP313370ENV
CSB-EP313370ENV
CSB-BP313370ENV
CSB-MP313370ENV
CSB-EP313370ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drymarchon corais erebennus Hemoglobin subunit alpha-D CSB-YP313371DHD
CSB-EP313371DHD
CSB-BP313371DHD
CSB-MP313371DHD
CSB-EP313371DHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Diphthamide biosynthesis protein 4 (dph4) CSB-YP313372EKF
CSB-EP313372EKF
CSB-BP313372EKF
CSB-MP313372EKF
CSB-EP313372EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri Dimethylamine methyltransferase mtbB1 (mtbB1) CSB-YP313373MSM
CSB-EP313373MSM
CSB-BP313373MSM
CSB-MP313373MSM
CSB-EP313373MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Light-harvesting protein B-875 alpha chain (pufA), partial CSB-YP313374RLE
CSB-EP313374RLE
CSB-BP313374RLE
CSB-MP313374RLE
CSB-EP313374RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L16 (rplP) CSB-YP313375BMN
CSB-EP313375BMN
CSB-BP313375BMN
CSB-MP313375BMN
CSB-EP313375BMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S15 (rpsO) CSB-YP313376BMN
CSB-EP313376BMN
CSB-BP313376BMN
CSB-MP313376BMN
CSB-EP313376BMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 3-dehydroquinate dehydratase (aroQ) CSB-YP313377BWS
CSB-EP313377BWS
CSB-BP313377BWS
CSB-MP313377BWS
CSB-EP313377BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Probable periplasmic serine endoprotease DegP-like (htrA) CSB-YP313378BWS
CSB-EP313378BWS
CSB-BP313378BWS
CSB-MP313378BWS
CSB-EP313378BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Immunogenic membrane protein YajC (yajC), partial CSB-YP313379BWS1
CSB-EP313379BWS1
CSB-BP313379BWS1
CSB-MP313379BWS1
CSB-EP313379BWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Isoleucine--tRNA ligase (ileS), partial CSB-YP313380BMN
CSB-EP313380BMN
CSB-BP313380BMN
CSB-MP313380BMN
CSB-EP313380BMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin-responsive protein IAA27 (IAA27) CSB-YP313381OFG
CSB-EP313381OFG
CSB-BP313381OFG
CSB-MP313381OFG
CSB-EP313381OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Non-structural protein 1 (NS) CSB-YP313382IKF
CSB-EP313382IKF
CSB-BP313382IKF
CSB-MP313382IKF
CSB-EP313382IKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Acetate non-utilizing protein 9, mitochondrial (ACN9) CSB-YP313383MPM
CSB-EP313383MPM
CSB-BP313383MPM
CSB-MP313383MPM
CSB-EP313383MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Serine/threonine-protein phosphatase 2A activator 2 (rrd2) CSB-YP313384EKF
CSB-EP313384EKF
CSB-BP313384EKF
CSB-MP313384EKF
CSB-EP313384EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Photosystem I assembly protein ycf3 (ycf3) CSB-YP313385SAN
CSB-EP313385SAN
CSB-BP313385SAN
CSB-MP313385SAN
CSB-EP313385SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides limbatus Potassium channel toxin alpha-KTx 1.4 CSB-YP313386CFZ
CSB-EP313386CFZ
CSB-BP313386CFZ
CSB-MP313386CFZ
CSB-EP313386CFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP313387AJN
CSB-EP313387AJN
CSB-BP313387AJN
CSB-MP313387AJN
CSB-EP313387AJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyruvate-flavodoxin oxidoreductase (nifJ) CSB-YP313388AJN
CSB-EP313388AJN
CSB-BP313388AJN
CSB-MP313388AJN
CSB-EP313388AJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>