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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anterhynchium flavomarginatum micado Eumenine mastoparan-AF CSB-YP313348ALD
CSB-EP313348ALD
CSB-BP313348ALD
CSB-MP313348ALD
CSB-EP313348ALD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ubiquitin-like protein-NEDD8-like protein RUB3 (RUB3) CSB-YP313349OFG
CSB-EP313349OFG
CSB-BP313349OFG
CSB-MP313349OFG
CSB-EP313349OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Type VII secretion system extracellular protein A (esxA) CSB-YP313350FLG
CSB-BP313350FLG
CSB-MP313350FLG
CSB-EP313350FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Type VII secretion system extracellular protein C (esxC) CSB-YP313351FLG
CSB-BP313351FLG
CSB-MP313351FLG
CSB-EP313351FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP313352ENV
CSB-EP313352ENV
CSB-BP313352ENV
CSB-MP313352ENV
CSB-EP313352ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Multidrug/solvent efflux pump outer membrane protein mepC (mepC) CSB-YP313353FFZ
CSB-EP313353FFZ
CSB-BP313353FFZ
CSB-MP313353FFZ
CSB-EP313353FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli mRNA interferase RelE (relE) CSB-YP313354ENV
CSB-EP313354ENV
CSB-BP313354ENV
CSB-MP313354ENV
CSB-EP313354ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Leukotoxin (lktA), partial CSB-YP313355ESE
CSB-EP313355ESE
CSB-BP313355ESE
CSB-MP313355ESE
CSB-EP313355ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 3 (rpmG3) CSB-YP313356SKY
CSB-EP313356SKY
CSB-BP313356SKY
CSB-MP313356SKY
CSB-EP313356SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Putative sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP313357BXE
CSB-EP313357BXE
CSB-BP313357BXE
CSB-MP313357BXE
CSB-EP313357BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP313358SMT
CSB-EP313358SMT
CSB-BP313358SMT
CSB-MP313358SMT
CSB-EP313358SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major cold shock protein (cspA) CSB-YP313359SMR
CSB-EP313359SMR
CSB-BP313359SMR
CSB-MP313359SMR
CSB-EP313359SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroneopterin aldolase (folB) CSB-YP313360SMR
CSB-EP313360SMR
CSB-BP313360SMR
CSB-MP313360SMR
CSB-EP313360SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UTP--glucose-1-phosphate uridylyltransferase 1 (hasC1) CSB-YP313361SMT
CSB-EP313361SMT
CSB-BP313361SMT
CSB-MP313361SMT
CSB-EP313361SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyopneumoniae L-lactate dehydrogenase (ldh) CSB-YP313362MLQ
CSB-EP313362MLQ
CSB-BP313362MLQ
CSB-MP313362MLQ
CSB-EP313362MLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyopneumoniae Uncharacterized protein MHJ_0132 (MHJ_0132), partial CSB-YP313363MLQ
CSB-EP313363MLQ
CSB-BP313363MLQ
CSB-MP313363MLQ
CSB-EP313363MLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein iscX (iscX) CSB-YP313365ENV
CSB-EP313365ENV
CSB-BP313365ENV
CSB-MP313365ENV
CSB-EP313365ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP313366FLL
CSB-EP313366FLL
CSB-BP313366FLL
CSB-MP313366FLL
CSB-EP313366FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Extracellular elastase (sepA) CSB-YP313367FLL
CSB-EP313367FLL
CSB-BP313367FLL
CSB-MP313367FLL
CSB-EP313367FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarS (sarS) CSB-YP313368SUK
CSB-EP313368SUK
CSB-BP313368SUK
CSB-MP313368SUK
CSB-EP313368SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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