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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP313553OFG1
CSB-EP313553OFG1
CSB-BP313553OFG1
CSB-MP313553OFG1
CSB-EP313553OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Photosystem I reaction center subunit IX (psaJ), partial CSB-YP313554OCO
CSB-EP313554OCO
CSB-BP313554OCO
CSB-MP313554OCO
CSB-EP313554OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Translation initiation factor IF-1, chloroplastic (infA) CSB-YP313555OFG
CSB-EP313555OFG
CSB-BP313555OFG
CSB-MP313555OFG
CSB-EP313555OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP313556OFF
CSB-EP313556OFF
CSB-BP313556OFF
CSB-MP313556OFF
CSB-EP313556OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP313557OCO
CSB-EP313557OCO
CSB-BP313557OCO
CSB-MP313557OCO
CSB-EP313557OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Type IV secretion system protein virB1 (virB1) CSB-YP313558BWS
CSB-EP313558BWS
CSB-BP313558BWS
CSB-MP313558BWS
CSB-EP313558BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Photosystem II reaction center protein I (psbI), partial CSB-YP313559OCO
CSB-EP313559OCO
CSB-BP313559OCO
CSB-MP313559OCO
CSB-EP313559OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem II reaction center protein I (psbI), partial CSB-YP313560OFG
CSB-EP313560OFG
CSB-BP313560OFG
CSB-MP313560OFG
CSB-EP313560OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem II reaction center protein K (psbK) CSB-YP313561OFG
CSB-EP313561OFG
CSB-BP313561OFG
CSB-MP313561OFG
CSB-EP313561OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem II reaction center protein M (psbM), partial CSB-YP313562OFG
CSB-EP313562OFG
CSB-BP313562OFG
CSB-MP313562OFG
CSB-EP313562OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein psbN (psbN), partial CSB-YP313563OFF
CSB-EP313563OFF
CSB-BP313563OFF
CSB-MP313563OFF
CSB-EP313563OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Photosystem II reaction center protein H (psbH), partial CSB-YP313564OCO1
CSB-EP313564OCO1
CSB-BP313564OCO1
CSB-MP313564OCO1
CSB-EP313564OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Conotoxin mr3c CSB-YP313565DWD
CSB-EP313565DWD
CSB-BP313565DWD
CSB-MP313565DWD
CSB-EP313565DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Photosystem II reaction center protein Z (psbZ), partial CSB-YP313566OCO1
CSB-EP313566OCO1
CSB-BP313566OCO1
CSB-MP313566OCO1
CSB-EP313566OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Photosystem Q (B) protein, partial CSB-YP313567OCO1
CSB-EP313567OCO1
CSB-BP313567OCO1
CSB-MP313567OCO1
CSB-EP313567OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP313568OCO
CSB-EP313568OCO
CSB-BP313568OCO
CSB-MP313568OCO
CSB-EP313568OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP313569OCO
CSB-EP313569OCO
CSB-BP313569OCO
CSB-MP313569OCO
CSB-EP313569OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 50S ribosomal protein L23, chloroplastic (rpl23-A) CSB-YP313570OFF
CSB-EP313570OFF
CSB-BP313570OFF
CSB-MP313570OFF
CSB-EP313570OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP313571OFG
CSB-EP313571OFG
CSB-BP313571OFG
CSB-MP313571OFG
CSB-EP313571OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP313572OFF
CSB-EP313572OFF
CSB-BP313572OFF
CSB-MP313572OFF
CSB-EP313572OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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