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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Scolopendra viridicornis nigra Scolopendra 20417.15 Da toxin CSB-YP314570SDY
CSB-EP314570SDY
CSB-BP314570SDY
CSB-MP314570SDY
CSB-EP314570SDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Conantokin-Pr1 CSB-YP314571COZ
CSB-EP314571COZ
CSB-BP314571COZ
CSB-MP314571COZ
CSB-EP314571COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Conantokin-Pr3 CSB-YP314572COZ
CSB-EP314572COZ
CSB-BP314572COZ
CSB-MP314572COZ
CSB-EP314572COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus Evasin-4 CSB-YP314573RKI
CSB-EP314573RKI
CSB-BP314573RKI
CSB-MP314573RKI
CSB-EP314573RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Serine/threonine-protein kinase 1 (Pret-133) CSB-YP314574AYG
CSB-EP314574AYG
CSB-BP314574AYG
CSB-MP314574AYG
CSB-EP314574AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Serine/threonine-protein kinase 1 (Ken-133) CSB-YP314575AEB
CSB-EP314575AEB
CSB-BP314575AEB
CSB-MP314575AEB
CSB-EP314575AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Transcription factor S-II-related protein (Ken-151) CSB-YP314576AEB
CSB-EP314576AEB
CSB-BP314576AEB
CSB-MP314576AEB
CSB-EP314576AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Transcription factor S-II-related protein (Pret-152) CSB-YP314577AYG
CSB-EP314577AYG
CSB-BP314577AYG
CSB-MP314577AYG
CSB-EP314577AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Transcription factor S-II-related protein (War-150) CSB-YP314578AEI
CSB-EP314578AEI
CSB-BP314578AEI
CSB-MP314578AEI
CSB-EP314578AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Ubiquitin-conjugating enzyme E2 (UBC) CSB-YP314579AEB
CSB-EP314579AEB
CSB-BP314579AEB
CSB-MP314579AEB
CSB-EP314579AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Riboflavin biosynthesis protein ribF (ribF) CSB-YP314580EOD
CSB-EP314580EOD
CSB-BP314580EOD
CSB-MP314580EOD
CSB-EP314580EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP314581SZB
CSB-EP314581SZB
CSB-BP314581SZB
CSB-MP314581SZB
CSB-EP314581SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP314582EOD
CSB-EP314582EOD
CSB-BP314582EOD
CSB-MP314582EOD
CSB-EP314582EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14 (rpsN) CSB-YP314583EOD
CSB-EP314583EOD
CSB-BP314583EOD
CSB-MP314583EOD
CSB-EP314583EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA recombination protein rmuC (rmuC) CSB-YP314584EOD
CSB-EP314584EOD
CSB-BP314584EOD
CSB-MP314584EOD
CSB-EP314584EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfate-binding protein (sbp) CSB-YP314585ENV
CSB-EP314585ENV
CSB-BP314585ENV
CSB-MP314585ENV
CSB-EP314585ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase yghA (yghA) CSB-YP314586EOD
CSB-EP314586EOD
CSB-BP314586EOD
CSB-MP314586EOD
CSB-EP314586EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein translocase subunit SecD (secD), partial CSB-YP314587EOD1
CSB-EP314587EOD1
CSB-BP314587EOD1
CSB-MP314587EOD1
CSB-EP314587EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecY (secY), partial CSB-YP314588EOD
CSB-EP314588EOD
CSB-BP314588EOD
CSB-MP314588EOD
CSB-EP314588EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-E factor (rpoE) CSB-YP314589EOD
CSB-EP314589EOD
CSB-BP314589EOD
CSB-MP314589EOD
CSB-EP314589EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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