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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. indica Actin-7 (ACT7) CSB-YP313593OFF
CSB-EP313593OFF
CSB-BP313593OFF
CSB-MP313593OFF
CSB-EP313593OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cardiotoxin-like basic polypeptide ah CSB-YP313594NFG
CSB-EP313594NFG
CSB-BP313594NFG
CSB-MP313594NFG
CSB-EP313594NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus fasciatus Phospholipase A2 KBf-grIB CSB-YP313595BXL
CSB-EP313595BXL
CSB-BP313595BXL
CSB-MP313595BXL
CSB-EP313595BXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP313596MVW
CSB-EP313596MVW
CSB-BP313596MVW
CSB-MP313596MVW
CSB-EP313596MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP313597MVW
CSB-EP313597MVW
CSB-BP313597MVW
CSB-MP313597MVW
CSB-EP313597MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tanacetum cinerariifolium Chrysanthemyl diphosphate synthase, chloroplastic CSB-YP313598TAQ
CSB-EP313598TAQ
CSB-BP313598TAQ
CSB-MP313598TAQ
CSB-EP313598TAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mokola virus Large structural protein (L), partial CSB-YP313599MHF
CSB-EP313599MHF
CSB-BP313599MHF
CSB-MP313599MHF
CSB-EP313599MHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mokola virus Glycoprotein G (G), partial CSB-YP313600MHF1
CSB-EP313600MHF1
CSB-BP313600MHF1
CSB-MP313600MHF1
CSB-EP313600MHF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovophis okinavensis Thrombin-like enzyme okinaxobin-2 CSB-YP313601OCY
CSB-EP313601OCY
CSB-BP313601OCY
CSB-MP313601OCY
CSB-EP313601OCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Small COPII coat GTPase sar-1 (sar-1) CSB-YP313602NHA
CSB-EP313602NHA
CSB-BP313602NHA
CSB-MP313602NHA
CSB-EP313602NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutamyl-tRNA reductase, chloroplastic (Os10g0502400, LOC_Os10g35840) CSB-YP313603OFG
CSB-EP313603OFG
CSB-BP313603OFG
CSB-MP313603OFG
CSB-EP313603OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Non-structural protein of 12.7 kDa (5a) CSB-YP313605MJY
CSB-EP313605MJY
CSB-BP313605MJY
CSB-MP313605MJY
CSB-EP313605MJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica MADS-box transcription factor 14 (MADS14) CSB-YP313606OFF
CSB-EP313606OFF
CSB-BP313606OFF
CSB-MP313606OFF
CSB-EP313606OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Metallothionein-like protein 1A (MT1A) CSB-YP313607OFG
CSB-EP313607OFG
CSB-BP313607OFG
CSB-MP313607OFG
CSB-EP313607OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase-like 4 (4CLL4), partial CSB-YP313608DOA
CSB-EP313608DOA
CSB-BP313608DOA
CSB-MP313608DOA
CSB-EP313608DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyclopropane mycolic acid synthase 1 (cmaA1) CSB-YP313609MVZ
CSB-EP313609MVZ
CSB-BP313609MVZ
CSB-MP313609MVZ
CSB-EP313609MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 35 kDa protein (Rv2744c, MT2815) CSB-YP313610MVZ
CSB-EP313610MVZ
CSB-BP313610MVZ
CSB-MP313610MVZ
CSB-EP313610MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Glycine-rich cell wall structural protein 2 (GRP0.9) CSB-YP313611OFF
CSB-EP313611OFF
CSB-BP313611OFF
CSB-MP313611OFF
CSB-EP313611OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low molecular weight protein-tyrosine-phosphatase ptpA (ptpA) CSB-YP313612FKZ
CSB-EP313612FKZ
CSB-BP313612FKZ
CSB-MP313612FKZ
CSB-EP313612FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Serine/threonine-protein kinase SAPK3 (SAPK3) CSB-YP313613OFG
CSB-EP313613OFG
CSB-BP313613OFG
CSB-MP313613OFG
CSB-EP313613OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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