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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant African swine fever virus Protein DP71L (War-170) CSB-YP315182AEI
CSB-EP315182AEI
CSB-BP315182AEI
CSB-MP315182AEI
CSB-EP315182AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein DP71L (Pret-172) CSB-YP315183AYG
CSB-EP315183AYG
CSB-BP315183AYG
CSB-MP315183AYG
CSB-EP315183AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Puumala virus RNA-directed RNA polymerase L (L), partial CSB-YP315184PXI
CSB-EP315184PXI
CSB-BP315184PXI
CSB-MP315184PXI
CSB-EP315184PXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Protein W (W) CSB-YP315185NCT
CSB-EP315185NCT
CSB-BP315185NCT
CSB-MP315185NCT
CSB-EP315185NCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sudan ebolavirus Super small secreted glycoprotein (GP) CSB-YP315186SRE
CSB-EP315186SRE
CSB-BP315186SRE
CSB-MP315186SRE
CSB-EP315186SRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag-pro), partial CSB-YP315187ASN
CSB-BP315187ASN
CSB-MP315187ASN
CSB-EP315187ASN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactate dehydrogenase elevating virus Nucleoprotein (N) CSB-YP315188LAI
CSB-EP315188LAI
CSB-BP315188LAI
CSB-MP315188LAI
CSB-EP315188LAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X Movement protein TGB2 (ORF3), partial CSB-YP315189PRF1
CSB-EP315189PRF1
CSB-BP315189PRF1
CSB-MP315189PRF1
CSB-EP315189PRF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Protein PB1-F2 (PB1) CSB-YP315190IEF
CSB-EP315190IEF
CSB-BP315190IEF
CSB-MP315190IEF
CSB-EP315190IEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borna disease virus Matrix protein (M) CSB-YP315191BIM
CSB-EP315191BIM
CSB-BP315191BIM
CSB-MP315191BIM
CSB-EP315191BIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse KH homology domain-containing protein 1B (Khdc1b) CSB-YP315192MO
CSB-EP315192MO
CSB-BP315192MO
CSB-MP315192MO
CSB-EP315192MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echis leucogaster Disintegrin leucogastin-B CSB-YP315193EAJ
CSB-EP315193EAJ
CSB-BP315193EAJ
CSB-MP315193EAJ
CSB-EP315193EAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Antitoxin MazE (mazE) CSB-YP315194FLG
CSB-EP315194FLG
CSB-BP315194FLG
CSB-MP315194FLG
CSB-EP315194FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum RCC1 domain-containing protein DDB_G0295713 (DDB_G0295713), partial CSB-YP315195DKK
CSB-EP315195DKK
CSB-BP315195DKK
CSB-MP315195DKK
CSB-EP315195DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Inactive ubiquitin carboxyl-terminal hydrolase 17-like protein 8 (USP17L8) CSB-YP315196HU
CSB-EP315196HU
CSB-BP315196HU
CSB-MP315196HU
CSB-EP315196HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor rcnR (rcnR) CSB-YP315197EJE
CSB-EP315197EJE
CSB-BP315197EJE
CSB-MP315197EJE
CSB-EP315197EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (lpsJ) CSB-YP315198XAY
CSB-EP315198XAY
CSB-BP315198XAY
CSB-MP315198XAY
CSB-EP315198XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Uncharacterized protein wxcX (wxcX), partial CSB-YP315199XAY
CSB-EP315199XAY
CSB-BP315199XAY
CSB-MP315199XAY
CSB-EP315199XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase (paaJ) CSB-YP315200ENV
CSB-EP315200ENV
CSB-BP315200ENV
CSB-MP315200ENV
CSB-EP315200ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator B-115 (b115) CSB-YP315201SRM
CSB-EP315201SRM
CSB-BP315201SRM
CSB-MP315201SRM
CSB-EP315201SRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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