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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Vpr (vpr) CSB-YP314796HLP
CSB-BP314796HLP
CSB-MP314796HLP
CSB-EP314796HLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase III polC-type (polC), partial CSB-YP314797FKZ
CSB-EP314797FKZ
CSB-BP314797FKZ
CSB-MP314797FKZ
CSB-EP314797FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa tropica Mastoparan-T CSB-YP314798VBT
CSB-EP314798VBT
CSB-BP314798VBT
CSB-MP314798VBT
CSB-EP314798VBT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactose-6-phosphate isomerase subunit lacA (lacA) CSB-YP314799FKZ
CSB-EP314799FKZ
CSB-BP314799FKZ
CSB-MP314799FKZ
CSB-EP314799FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Uncharacterized BphX-like protein CSB-YP314800BXR
CSB-EP314800BXR
CSB-BP314800BXR
CSB-MP314800BXR
CSB-EP314800BXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Terebra subulata Augertoxin-s7a CSB-YP314801TBG
CSB-EP314801TBG
CSB-BP314801TBG
CSB-MP314801TBG
CSB-EP314801TBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 9 (GLU1), partial CSB-YP314802OFG
CSB-EP314802OFG
CSB-BP314802OFG
CSB-MP314802OFG
CSB-EP314802OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA topoisomerase 4 subunit A (parC), partial CSB-YP314803FKZ
CSB-EP314803FKZ
CSB-BP314803FKZ
CSB-MP314803FKZ
CSB-EP314803FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus radiatus Alpha-conotoxin RVIIIA CSB-YP314804CPD
CSB-EP314804CPD
CSB-BP314804CPD
CSB-MP314804CPD
CSB-EP314804CPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus pergrandis Alpha-conotoxin PeIVB CSB-YP314805CPB
CSB-EP314805CPB
CSB-BP314805CPB
CSB-MP314805CPB
CSB-EP314805CPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0387 membrane protein yohO (yohO), partial CSB-YP314806EGX
CSB-EP314806EGX
CSB-BP314806EGX
CSB-MP314806EGX
CSB-EP314806EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tachypleus tridentatus Tachystatin-A1 CSB-YP314807TAH
CSB-EP314807TAH
CSB-BP314807TAH
CSB-MP314807TAH
CSB-EP314807TAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (tax) CSB-YP314808HQI
CSB-EP314808HQI
CSB-BP314808HQI
CSB-MP314808HQI
CSB-EP314808HQI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psalmopoeus cambridgei Tau-theraphotoxin-Pc1b CSB-YP314809PSZ
CSB-EP314809PSZ
CSB-BP314809PSZ
CSB-MP314809PSZ
CSB-EP314809PSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus emaciatus Kappa-conotoxin-like Em11.10 CSB-YP314810DVZ
CSB-EP314810DVZ
CSB-BP314810DVZ
CSB-MP314810DVZ
CSB-EP314810DVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized serine/threonine-protein kinase SgK110 (SGK110) CSB-YP314811HU
CSB-EP314811HU
CSB-BP314811HU
CSB-MP314811HU
CSB-EP314811HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yibT (yibT) CSB-YP314812SZB
CSB-EP314812SZB
CSB-BP314812SZB
CSB-MP314812SZB
CSB-EP314812SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YOL013W-A (YOL013W-A) CSB-YP314813SVG
CSB-EP314813SVG
CSB-BP314813SVG
CSB-MP314813SVG
CSB-EP314813SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate reductase flavoprotein subunit (fccA), partial CSB-YP314814SFY
CSB-EP314814SFY
CSB-BP314814SFY
CSB-MP314814SFY
CSB-EP314814SFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Monothiol glutaredoxin-S9 (GRXS9) CSB-YP314815OFG
CSB-EP314815OFG
CSB-BP314815OFG
CSB-MP314815OFG
CSB-EP314815OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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