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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Epstein-Barr virus Apoptosis regulator BALF1 (BALF1) CSB-YP316796EFA
CSB-EP316796EFA
CSB-BP316796EFA
CSB-MP316796EFA
CSB-EP316796EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CFA/I fimbrial subunit B (cfaB) CSB-YP316797ENL
CSB-BP316797ENL
CSB-MP316797ENL
CSB-EP316797ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cold shock-like protein CspJ (cspJ) CSB-YP316798SXB
CSB-EP316798SXB
CSB-BP316798SXB
CSB-MP316798SXB
CSB-EP316798SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Endonuclease 8 (nei) CSB-YP316799SXB
CSB-EP316799SXB
CSB-BP316799SXB
CSB-MP316799SXB
CSB-EP316799SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Transcriptional regulator hilD (hilD) CSB-YP316800SXB
CSB-EP316800SXB
CSB-BP316800SXB
CSB-MP316800SXB
CSB-EP316800SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium HTH-type transcriptional regulator mlrA (mlrA) CSB-YP316801SXB
CSB-EP316801SXB
CSB-BP316801SXB
CSB-MP316801SXB
CSB-EP316801SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chaperone protein sicP (sicP) CSB-YP316802SXB
CSB-EP316802SXB
CSB-BP316802SXB
CSB-MP316802SXB
CSB-EP316802SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0377 protein YIL175W (YIL175W) CSB-YP316803SVG
CSB-EP316803SVG
CSB-BP316803SVG
CSB-MP316803SVG
CSB-EP316803SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YHR054W-A (YHR054W-A), partial CSB-YP316804SVG1
CSB-EP316804SVG1
CSB-BP316804SVG1
CSB-MP316804SVG1
CSB-EP316804SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0479 protein YOR396C-A (YOR396C-A), partial CSB-YP316805SVG1
CSB-EP316805SVG1
CSB-BP316805SVG1
CSB-MP316805SVG1
CSB-EP316805SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ferrioxamine B receptor (foxA), partial CSB-YP316806SXB
CSB-EP316806SXB
CSB-BP316806SXB
CSB-MP316806SXB
CSB-EP316806SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae S- (hydroxymethyl)glutathione dehydrogenase CSB-YP316807DPA
CSB-EP316807DPA
CSB-BP316807DPA
CSB-MP316807DPA
CSB-EP316807DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Antitoxin RelB3 (relB3) CSB-YP316808MRU
CSB-EP316808MRU
CSB-BP316808MRU
CSB-MP316808MRU
CSB-EP316808MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin MazE9 (mazE9) CSB-YP316809MVZ
CSB-EP316809MVZ
CSB-BP316809MVZ
CSB-MP316809MVZ
CSB-EP316809MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Outer surface 22 kDa lipoprotein (p22) CSB-YP316810BUD
CSB-EP316810BUD
CSB-BP316810BUD
CSB-MP316810BUD
CSB-EP316810BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Glutamate dehydrogenase (gdhA) CSB-YP316811FHX
CSB-EP316811FHX
CSB-BP316811FHX
CSB-MP316811FHX
CSB-EP316811FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase 1 (polI), partial CSB-YP316812PXQ
CSB-EP316812PXQ
CSB-BP316812PXQ
CSB-MP316812PXQ
CSB-EP316812PXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Iron-sulfur clusters transporter ATM1, mitochondrial (ATM1), partial CSB-YP316813CTL1
CSB-EP316813CTL1
CSB-BP316813CTL1
CSB-MP316813CTL1
CSB-EP316813CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-rRNA-processing protein ESF2 (ESF2) CSB-YP316814CTL
CSB-EP316814CTL
CSB-BP316814CTL
CSB-MP316814CTL
CSB-EP316814CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Acetate non-utilizing protein 9, mitochondrial (ACN9) CSB-YP316815CTL
CSB-EP316815CTL
CSB-BP316815CTL
CSB-MP316815CTL
CSB-EP316815CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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