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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida albicans Lipoyl synthase, mitochondrial (LAB5) CSB-YP315584CZE
CSB-EP315584CZE
CSB-BP315584CZE
CSB-MP315584CZE
CSB-EP315584CZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Increased recombination centers protein 22-2 (IRC22-2), partial CSB-YP315585CZE
CSB-EP315585CZE
CSB-BP315585CZE
CSB-MP315585CZE
CSB-EP315585CZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja kaouthia Cytotoxin NK-CT1 CSB-YP315586NAF
CSB-EP315586NAF
CSB-BP315586NAF
CSB-MP315586NAF
CSB-EP315586NAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombus terrestris Venom protease CSB-YP315587BIF
CSB-EP315587BIF
CSB-BP315587BIF
CSB-MP315587BIF
CSB-EP315587BIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ehrlichia chaffeensis Transcription termination factor Rho 1 (rho1) CSB-YP315588EJS
CSB-EP315588EJS
CSB-BP315588EJS
CSB-MP315588EJS
CSB-EP315588EJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Adenylosuccinate synthetase (ADE12) CSB-YP315589CZD
CSB-EP315589CZD
CSB-BP315589CZD
CSB-MP315589CZD
CSB-EP315589CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus tinianus Alpha-conotoxin CSB-YP315590CPK
CSB-EP315590CPK
CSB-BP315590CPK
CSB-MP315590CPK
CSB-EP315590CPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Impages hectica Toxin hheTx5 CSB-YP315591IAL
CSB-EP315591IAL
CSB-BP315591IAL
CSB-MP315591IAL
CSB-EP315591IAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Impages hectica Toxin hhe6.4 CSB-YP315592IAL
CSB-EP315592IAL
CSB-BP315592IAL
CSB-MP315592IAL
CSB-EP315592IAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus araneosus Chi-conotoxin-like Ar1248 CSB-YP315593CNW
CSB-EP315593CNW
CSB-BP315593CNW
CSB-MP315593CNW
CSB-EP315593CNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sordaria macrospora Peptidase M20 domain-containing protein SMAC_03666.2 (SMAC_03666.2) CSB-YP315594FIM
CSB-EP315594FIM
CSB-BP315594FIM
CSB-MP315594FIM
CSB-EP315594FIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Methionine import ATP-binding protein MetN (metN) CSB-YP315595LND
CSB-EP315595LND
CSB-BP315595LND
CSB-MP315595LND
CSB-EP315595LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Probable DHNTP pyrophosphohydrolase (folQ) CSB-YP315596LND
CSB-EP315596LND
CSB-BP315596LND
CSB-MP315596LND
CSB-EP315596LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin beta-KTx 14.3 CSB-YP315597LRM
CSB-EP315597LRM
CSB-BP315597LRM
CSB-MP315597LRM
CSB-EP315597LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Lipolysis-activating peptide 1-alpha chain CSB-YP315598LRM
CSB-EP315598LRM
CSB-BP315598LRM
CSB-MP315598LRM
CSB-EP315598LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin LmNaTx30 CSB-YP315599LRM
CSB-EP315599LRM
CSB-BP315599LRM
CSB-MP315599LRM
CSB-EP315599LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin LmNaTx11.6 CSB-YP315600LRM
CSB-EP315600LRM
CSB-BP315600LRM
CSB-MP315600LRM
CSB-EP315600LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Talaromyces stipitatus Catabolic 3-dehydroquinase (qutE) CSB-YP315601TKZ
CSB-EP315601TKZ
CSB-BP315601TKZ
CSB-MP315601TKZ
CSB-EP315601TKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YAR062W (YAR062W) CSB-YP315602SVG
CSB-EP315602SVG
CSB-BP315602SVG
CSB-MP315602SVG
CSB-EP315602SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium MgtA leader peptide (mgtL) CSB-YP315603SWY
CSB-EP315603SWY
CSB-BP315603SWY
CSB-MP315603SWY
CSB-EP315603SWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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