Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein TIF31 homolog (TIF31), partial CSB-YP315745CTL
CSB-EP315745CTL
CSB-BP315745CTL
CSB-MP315745CTL
CSB-EP315745CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D GDP-mannose transporter 1 (GMT1), partial CSB-YP315746CTL
CSB-EP315746CTL
CSB-BP315746CTL
CSB-MP315746CTL
CSB-EP315746CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (adenine (58)-N (1))-methyltransferase non-catalytic subunit TRM6, partial CSB-YP315747CTK
CSB-EP315747CTK
CSB-BP315747CTK
CSB-MP315747CTK
CSB-EP315747CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UPF0390 protein CNBD1430 (CNBD1430) CSB-YP315748CTK
CSB-EP315748CTK
CSB-BP315748CTK
CSB-MP315748CTK
CSB-EP315748CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D WD repeat-containing protein JIP5 (JIP5) CSB-YP315749CTL
CSB-EP315749CTL
CSB-BP315749CTL
CSB-MP315749CTL
CSB-EP315749CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Polyadenylation factor subunit 2 (PFS2), partial CSB-YP315750CTL
CSB-EP315750CTL
CSB-BP315750CTL
CSB-MP315750CTL
CSB-EP315750CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Polyadenylation factor subunit 2 (PFS2), partial CSB-YP315751CTK
CSB-EP315751CTK
CSB-BP315751CTK
CSB-MP315751CTK
CSB-EP315751CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (guanine-N (7)-)-methyltransferase subunit TRM82, partial CSB-YP315752CTL
CSB-EP315752CTL
CSB-BP315752CTL
CSB-MP315752CTL
CSB-EP315752CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D WD repeat-containing protein CNBH2930 (CNBH2930), partial CSB-YP315753CTK
CSB-EP315753CTK
CSB-BP315753CTK
CSB-MP315753CTK
CSB-EP315753CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Palmitoyltransferase AKR1 (AKR1), partial CSB-YP315754CTK1
CSB-EP315754CTK1
CSB-BP315754CTK1
CSB-MP315754CTK1
CSB-EP315754CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (guanine-N (7)-)-methyltransferase CSB-YP315755CTL
CSB-EP315755CTL
CSB-BP315755CTL
CSB-MP315755CTL
CSB-EP315755CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein CPT2R1.03 (SPBCPT2R1.03) CSB-YP315756SXV
CSB-EP315756SXV
CSB-BP315756SXV
CSB-MP315756SXV
CSB-EP315756SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1954A (Rv1954A) CSB-YP315757MVZ
CSB-EP315757MVZ
CSB-BP315757MVZ
CSB-MP315757MVZ
CSB-EP315757MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Peroxiredoxin-4 CSB-YP315758DYC
CSB-EP315758DYC
CSB-BP315758DYC
CSB-MP315758DYC
CSB-EP315758DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa (3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial, partial CSB-YP315759FEZ
CSB-EP315759FEZ
CSB-BP315759FEZ
CSB-MP315759FEZ
CSB-EP315759FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Testis-specific Y-encoded protein 8 (TSPY8) CSB-YP315760HU
CSB-EP315760HU
CSB-BP315760HU
CSB-MP315760HU
CSB-EP315760HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Acetate kinase (ackA) CSB-YP315761MFC
CSB-EP315761MFC
CSB-BP315761MFC
CSB-MP315761MFC
CSB-EP315761MFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LIM and senescent cell antigen-like-containing domain protein 3-like (LIMS3L) CSB-YP315763HU
CSB-EP315763HU
CSB-BP315763HU
CSB-MP315763HU
CSB-EP315763HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB1 (vapB1) CSB-YP315764MVZ
CSB-EP315764MVZ
CSB-BP315764MVZ
CSB-MP315764MVZ
CSB-EP315764MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB36 (vapB36) CSB-YP315765MVZ
CSB-EP315765MVZ
CSB-BP315765MVZ
CSB-MP315765MVZ
CSB-EP315765MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>