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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Pentafunctional AROM polypeptide (CNB01990), partial CSB-YP315665CTL
CSB-EP315665CTL
CSB-BP315665CTL
CSB-MP315665CTL
CSB-EP315665CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATPase GET3 (GET3) CSB-YP315666CTK
CSB-EP315666CTK
CSB-BP315666CTK
CSB-MP315666CTK
CSB-EP315666CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 2 (ATG2), partial CSB-YP315667CTL
CSB-EP315667CTL
CSB-BP315667CTL
CSB-MP315667CTL
CSB-EP315667CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 3 (ATG3) CSB-YP315668CTK
CSB-EP315668CTK
CSB-BP315668CTK
CSB-MP315668CTK
CSB-EP315668CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein CFT1 (CFT1), partial CSB-YP315669CTK
CSB-EP315669CTK
CSB-BP315669CTK
CSB-MP315669CTK
CSB-EP315669CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D rRNA-processing protein CGR1 (CGR1) CSB-YP315670CTL
CSB-EP315670CTL
CSB-BP315670CTL
CSB-MP315670CTL
CSB-EP315670CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Chitin synthase export chaperone (CHS7), partial CSB-YP315671CTK1
CSB-EP315671CTK1
CSB-BP315671CTK1
CSB-MP315671CTK1
CSB-EP315671CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein CLP1 (CLP1), partial CSB-YP315672CTL
CSB-EP315672CTL
CSB-BP315672CTL
CSB-MP315672CTL
CSB-EP315672CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4), partial CSB-YP315673CTK
CSB-EP315673CTK
CSB-BP315673CTK
CSB-MP315673CTK
CSB-EP315673CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CWC22 (CWC22), partial CSB-YP315674CTL
CSB-EP315674CTL
CSB-BP315674CTL
CSB-MP315674CTL
CSB-EP315674CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CWC25 (CWC25) CSB-YP315675CTL
CSB-EP315675CTL
CSB-BP315675CTL
CSB-MP315675CTL
CSB-EP315675CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cyanate hydratase (CYN1) CSB-YP315676CTK
CSB-EP315676CTK
CSB-BP315676CTK
CSB-MP315676CTK
CSB-EP315676CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Defective in cullin neddylation protein 1 (DCN1) CSB-YP315677CTK
CSB-EP315677CTK
CSB-BP315677CTK
CSB-MP315677CTK
CSB-EP315677CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein YOP1 (YOP1), partial CSB-YP315678CTK1
CSB-EP315678CTK1
CSB-BP315678CTK1
CSB-MP315678CTK1
CSB-EP315678CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D DNA polymerase epsilon subunit B (DPB2), partial CSB-YP315679CTL
CSB-EP315679CTL
CSB-BP315679CTL
CSB-MP315679CTL
CSB-EP315679CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Elongation factor 1-alpha (TEF1) CSB-YP315680CTK
CSB-EP315680CTK
CSB-BP315680CTK
CSB-MP315680CTK
CSB-EP315680CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein EFR3 (EFR3), partial CSB-YP315681CTK
CSB-EP315681CTK
CSB-BP315681CTK
CSB-MP315681CTK
CSB-EP315681CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Hsp70 nucleotide exchange factor FES1 (FES1) CSB-YP315682CTK
CSB-EP315682CTK
CSB-BP315682CTK
CSB-MP315682CTK
CSB-EP315682CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Endoplasmic reticulum vesicle protein 25 (ERV25), partial CSB-YP315683CTL
CSB-EP315683CTL
CSB-BP315683CTL
CSB-MP315683CTL
CSB-EP315683CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 1,4-alpha-glucan-branching enzyme (GLC3), partial CSB-YP315684CTK
CSB-EP315684CTK
CSB-BP315684CTK
CSB-MP315684CTK
CSB-EP315684CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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