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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Nuclear protein localization protein 4 (NPL4), partial CSB-YP315705CTK
CSB-EP315705CTK
CSB-BP315705CTK
CSB-MP315705CTK
CSB-EP315705CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Stress response protein NST1 (NST1), partial CSB-YP315706CTL
CSB-EP315706CTL
CSB-BP315706CTL
CSB-MP315706CTL
CSB-EP315706CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Presequence translocated-associated motor subunit PAM17, mitochondrial (PAM17), partial CSB-YP315707CTL1
CSB-EP315707CTL1
CSB-BP315707CTL1
CSB-MP315707CTL1
CSB-EP315707CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D PAB-dependent poly (A)-specific ribonuclease subunit PAN3, partial CSB-YP315708CTL
CSB-EP315708CTL
CSB-BP315708CTL
CSB-MP315708CTL
CSB-EP315708CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Serine/threonine-protein kinase TEL1 (TEL1), partial CSB-YP315709CTL
CSB-EP315709CTL
CSB-BP315709CTL
CSB-MP315709CTL
CSB-EP315709CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D GPI-anchored wall transfer protein 1 (GWT1), partial CSB-YP315710CTK
CSB-EP315710CTK
CSB-BP315710CTK
CSB-MP315710CTK
CSB-EP315710CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP315711CTK
CSB-EP315711CTK
CSB-BP315711CTK
CSB-MP315711CTK
CSB-EP315711CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Phospholipase B (PLB1), partial CSB-YP315712CTK
CSB-EP315712CTK
CSB-BP315712CTK
CSB-MP315712CTK
CSB-EP315712CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Peptidyl-prolyl isomerase CWC27 (CWC27) CSB-YP315713CTK
CSB-EP315713CTK
CSB-BP315713CTK
CSB-MP315713CTK
CSB-EP315713CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D FK506-binding protein 2 (FPR2) CSB-YP315714CTK
CSB-EP315714CTK
CSB-BP315714CTK
CSB-MP315714CTK
CSB-EP315714CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Serine/threonine-protein phosphatase 2A activator 2 (RRD2) CSB-YP315715CTK
CSB-EP315715CTK
CSB-BP315715CTK
CSB-MP315715CTK
CSB-EP315715CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cysteine protease ATG4 (ATG4), partial CSB-YP315716CTK
CSB-EP315716CTK
CSB-BP315716CTK
CSB-MP315716CTK
CSB-EP315716CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 (KAE1) CSB-YP315717CTL
CSB-EP315717CTL
CSB-BP315717CTL
CSB-MP315717CTL
CSB-EP315717CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial intermediate peptidase 2 (OCT2), partial CSB-YP315718CTL
CSB-EP315718CTL
CSB-BP315718CTL
CSB-MP315718CTL
CSB-EP315718CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Phosphoribosylaminoimidazole carboxylase (ADE2), partial CSB-YP315719CTL
CSB-EP315719CTL
CSB-BP315719CTL
CSB-MP315719CTL
CSB-EP315719CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SYM1 (SYM1), partial CSB-YP315720CTL1
CSB-EP315720CTL1
CSB-BP315720CTL1
CSB-MP315720CTL1
CSB-EP315720CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) CSB-YP315721CTK
CSB-EP315721CTK
CSB-BP315721CTK
CSB-MP315721CTK
CSB-EP315721CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SEY1 (SEY1), partial CSB-YP315722CTK
CSB-EP315722CTK
CSB-BP315722CTK
CSB-MP315722CTK
CSB-EP315722CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 13 kDa ribonucleoprotein-associated protein (SNU13) CSB-YP315723CTL
CSB-EP315723CTL
CSB-BP315723CTL
CSB-MP315723CTL
CSB-EP315723CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 40S ribosomal protein S0 (RPS0) CSB-YP315724CTL
CSB-EP315724CTL
CSB-BP315724CTL
CSB-MP315724CTL
CSB-EP315724CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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