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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Calcium-binding mitochondrial carrier SAL1 (SAL1), partial CSB-YP316756SAC1
CSB-EP316756SAC1
CSB-BP316756SAC1
CSB-MP316756SAC1
CSB-EP316756SAC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin alpha-KTx 12.7 CSB-YP316757LRM
CSB-EP316757LRM
CSB-BP316757LRM
CSB-MP316757LRM
CSB-EP316757LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin LmNaTx19 CSB-YP316758LRM
CSB-EP316758LRM
CSB-BP316758LRM
CSB-MP316758LRM
CSB-EP316758LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin LmNaTx35.2 CSB-YP316759LRM
CSB-EP316759LRM
CSB-BP316759LRM
CSB-MP316759LRM
CSB-EP316759LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Talaromyces stipitatus Quinate repressor protein (qutR), partial CSB-YP316760TKZ
CSB-EP316760TKZ
CSB-BP316760TKZ
CSB-MP316760TKZ
CSB-EP316760TKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YHR213W (YHR213W) CSB-YP316761SVG
CSB-EP316761SVG
CSB-BP316761SVG
CSB-MP316761SVG
CSB-EP316761SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Dyslexia-associated protein KIAA0319 homolog, partial CSB-YP316762RA
CSB-EP316762RA
CSB-BP316762RA
CSB-MP316762RA
CSB-EP316762RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin LmNaTx45.2 CSB-YP316763LRM
CSB-EP316763LRM
CSB-BP316763LRM
CSB-MP316763LRM
CSB-EP316763LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Putative neurotoxin-G CSB-YP316764LRM
CSB-EP316764LRM
CSB-BP316764LRM
CSB-MP316764LRM
CSB-EP316764LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Ponericin-W-like 32.1 CSB-YP316765LRM
CSB-EP316765LRM
CSB-BP316765LRM
CSB-MP316765LRM
CSB-EP316765LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Anionic peptide 17.1 CSB-YP316766LRM
CSB-EP316766LRM
CSB-BP316766LRM
CSB-MP316766LRM
CSB-EP316766LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurtoxin 10 CSB-YP316767LRM
CSB-EP316767LRM
CSB-BP316767LRM
CSB-MP316767LRM
CSB-EP316767LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 29 CSB-YP316768LRM
CSB-EP316768LRM
CSB-BP316768LRM
CSB-MP316768LRM
CSB-EP316768LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 3.1 CSB-YP316769LRM
CSB-EP316769LRM
CSB-BP316769LRM
CSB-MP316769LRM
CSB-EP316769LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 49.1 CSB-YP316770LRM
CSB-EP316770LRM
CSB-BP316770LRM
CSB-MP316770LRM
CSB-EP316770LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 9.1 CSB-YP316771LRM
CSB-EP316771LRM
CSB-BP316771LRM
CSB-MP316771LRM
CSB-EP316771LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Conotoxin Gla (2)-TxVI/B CSB-YP316772DWJ
CSB-EP316772DWJ
CSB-BP316772DWJ
CSB-MP316772DWJ
CSB-EP316772DWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus scutellatus scutellatus Taicatoxin, alpha-neurotoxin-like component CSB-YP316773OGF
CSB-EP316773OGF
CSB-BP316773OGF
CSB-MP316773OGF
CSB-EP316773OGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eumenes fraterculus Eumenine mastoparan-EF CSB-YP316774EHZ
CSB-EP316774EHZ
CSB-BP316774EHZ
CSB-MP316774EHZ
CSB-EP316774EHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chlorophyll a-b binding protein 2, chloroplastic (LHCB1.1), partial CSB-YP316775DOA1
CSB-EP316775DOA1
CSB-BP316775DOA1
CSB-MP316775DOA1
CSB-EP316775DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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