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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribosome biogenesis protein NSA2 (NSA2) CSB-YP315725CTL
CSB-EP315725CTL
CSB-BP315725CTL
CSB-MP315725CTL
CSB-EP315725CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D E3 ubiquitin-protein ligase BRE1 (BRE1), partial CSB-YP315726CTK
CSB-EP315726CTK
CSB-BP315726CTK
CSB-MP315726CTK
CSB-EP315726CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase FAL1 (FAL1) CSB-YP315727CTL
CSB-EP315727CTL
CSB-BP315727CTL
CSB-MP315727CTL
CSB-EP315727CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase ded1 (DED1), partial CSB-YP315728CTK
CSB-EP315728CTK
CSB-BP315728CTK
CSB-MP315728CTK
CSB-EP315728CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP315729CTL
CSB-EP315729CTL
CSB-BP315729CTL
CSB-MP315729CTL
CSB-EP315729CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase HAS1 (HAS1), partial CSB-YP315730CTK
CSB-EP315730CTK
CSB-BP315730CTK
CSB-MP315730CTK
CSB-EP315730CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase MAK5 (MAK5), partial CSB-YP315731CTL
CSB-EP315731CTL
CSB-BP315731CTL
CSB-MP315731CTL
CSB-EP315731CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP7 (DBP7), partial CSB-YP315732CTK
CSB-EP315732CTK
CSB-BP315732CTK
CSB-MP315732CTK
CSB-EP315732CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (PRP5), partial CSB-YP315733CTK
CSB-EP315733CTK
CSB-BP315733CTK
CSB-MP315733CTK
CSB-EP315733CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase ROK1 (ROK1), partial CSB-YP315734CTL
CSB-EP315734CTL
CSB-BP315734CTL
CSB-MP315734CTL
CSB-EP315734CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP9 (DBP9), partial CSB-YP315735CTK
CSB-EP315735CTK
CSB-BP315735CTK
CSB-MP315735CTK
CSB-EP315735CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Multiple RNA-binding domain-containing protein 1 (MRD1), partial CSB-YP315736CTK
CSB-EP315736CTK
CSB-BP315736CTK
CSB-MP315736CTK
CSB-EP315736CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D rRNA biogenesis protein RRP36 (RRP36) CSB-YP315737CTL
CSB-EP315737CTL
CSB-BP315737CTL
CSB-MP315737CTL
CSB-EP315737CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Small COPII coat GTPase SAR1 (SAR1) CSB-YP315738CTL
CSB-EP315738CTL
CSB-BP315738CTL
CSB-MP315738CTL
CSB-EP315738CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 3-ketoacyl-CoA reductase (CNBH0660), partial CSB-YP315739CTK1
CSB-EP315739CTK1
CSB-BP315739CTK1
CSB-MP315739CTK1
CSB-EP315739CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Potential protein lysine methyltransferase SET5 (SET5) CSB-YP315740CTK
CSB-EP315740CTK
CSB-BP315740CTK
CSB-MP315740CTK
CSB-EP315740CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein CSN12 homolog (CSN12) CSB-YP315741CTL
CSB-EP315741CTL
CSB-BP315741CTL
CSB-MP315741CTL
CSB-EP315741CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-processing protein 45 (PRP45), partial CSB-YP315742CTK
CSB-EP315742CTK
CSB-BP315742CTK
CSB-MP315742CTK
CSB-EP315742CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable kinetochore protein SPC25 (SPC25) CSB-YP315743CTK
CSB-EP315743CTK
CSB-BP315743CTK
CSB-MP315743CTK
CSB-EP315743CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Class E vacuolar protein-sorting machinery protein HSE1 (HSE1), partial CSB-YP315744CTL
CSB-EP315744CTL
CSB-BP315744CTL
CSB-MP315744CTL
CSB-EP315744CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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