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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Enolase-related protein 1 (ERR1) CSB-YP317833SVG
CSB-EP317833SVG
CSB-BP317833SVG
CSB-MP317833SVG
CSB-EP317833SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L43-B (RPL43B) CSB-YP317835SVG
CSB-EP317835SVG
CSB-BP317835SVG
CSB-MP317835SVG
CSB-EP317835SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S6-A (RPS6A) CSB-YP317836SVG
CSB-EP317836SVG
CSB-BP317836SVG
CSB-MP317836SVG
CSB-EP317836SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L23-B (RPL23B) CSB-YP317837SVG
CSB-EP317837SVG
CSB-BP317837SVG
CSB-MP317837SVG
CSB-EP317837SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L2-A (RPL2A) CSB-YP317838SVG
CSB-EP317838SVG
CSB-BP317838SVG
CSB-MP317838SVG
CSB-EP317838SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S11-B (RPS11B) CSB-YP317839SVG
CSB-EP317839SVG
CSB-BP317839SVG
CSB-MP317839SVG
CSB-EP317839SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S16-A (RPS16A) CSB-YP317840SVG
CSB-EP317840SVG
CSB-BP317840SVG
CSB-MP317840SVG
CSB-EP317840SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S18-B (RPS18B) CSB-YP317841SVG
CSB-EP317841SVG
CSB-BP317841SVG
CSB-MP317841SVG
CSB-EP317841SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-PR1 Gag polyprotein (TY1A-PR1) CSB-YP317842SVG
CSB-EP317842SVG
CSB-BP317842SVG
CSB-MP317842SVG
CSB-EP317842SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty2-GR2 Gag polyprotein (TY2A-GR2) CSB-YP317843SVG
CSB-EP317843SVG
CSB-BP317843SVG
CSB-MP317843SVG
CSB-EP317843SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-ER2 Gag polyprotein (TY1A-ER2) CSB-YP317844SVG
CSB-EP317844SVG
CSB-BP317844SVG
CSB-MP317844SVG
CSB-EP317844SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-LR3 Gag polyprotein (TY1A-LR3) CSB-YP317845SVG
CSB-EP317845SVG
CSB-BP317845SVG
CSB-MP317845SVG
CSB-EP317845SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Copper metallothionein 1-1 (CUP1-1) CSB-YP317846SVG
CSB-BP317846SVG
CSB-MP317846SVG
CSB-EP317846SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L35-A (RPL35A) CSB-YP317847SVG
CSB-EP317847SVG
CSB-BP317847SVG
CSB-MP317847SVG
CSB-EP317847SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YAR060C (YAR060C) CSB-YP317848SVG
CSB-EP317848SVG
CSB-BP317848SVG
CSB-MP317848SVG
CSB-EP317848SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0479 membrane protein YER190C-B (YER190C-B), partial CSB-YP317849SVG1
CSB-EP317849SVG1
CSB-BP317849SVG1
CSB-MP317849SVG1
CSB-EP317849SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0479 membrane protein YPL283W-B (YPL283W-B), partial CSB-YP317850SVG1
CSB-EP317850SVG1
CSB-BP317850SVG1
CSB-MP317850SVG1
CSB-EP317850SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0479 membrane protein YLL066W-A (YLL066W-A), partial CSB-YP317851SVG1
CSB-EP317851SVG1
CSB-BP317851SVG1
CSB-MP317851SVG1
CSB-EP317851SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YLR159C-A (YLR159C-A) CSB-YP317852SVG
CSB-EP317852SVG
CSB-BP317852SVG
CSB-MP317852SVG
CSB-EP317852SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Silenced mating-type protein ALPHA2 (HMLALPHA2) CSB-YP317853SVG
CSB-EP317853SVG
CSB-BP317853SVG
CSB-MP317853SVG
CSB-EP317853SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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