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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP317019SMV
CSB-EP317019SMV
CSB-BP317019SMV
CSB-MP317019SMV
CSB-EP317019SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized acetyltransferase SpyM3_1195 (SpyM3_1195) CSB-YP317020SMV
CSB-EP317020SMV
CSB-BP317020SMV
CSB-MP317020SMV
CSB-EP317020SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Translation initiation factor IF-1 (infA) CSB-YP317021SMW
CSB-EP317021SMW
CSB-BP317021SMW
CSB-MP317021SMW
CSB-EP317021SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP317022SMV
CSB-EP317022SMV
CSB-BP317022SMV
CSB-MP317022SMV
CSB-EP317022SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Galactose-6-phosphate isomerase subunit lacA 2 (lacA2) CSB-YP317023SMW
CSB-EP317023SMW
CSB-BP317023SMW
CSB-MP317023SMW
CSB-EP317023SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Galactose-6-phosphate isomerase subunit lacB 2 (lacB2) CSB-YP317024SMW
CSB-EP317024SMW
CSB-BP317024SMW
CSB-MP317024SMW
CSB-EP317024SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose 1,6-diphosphate aldolase 1 (lacD1) CSB-YP317025SMV
CSB-EP317025SMV
CSB-BP317025SMV
CSB-MP317025SMV
CSB-EP317025SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose 1,6-diphosphate aldolase 2 (lacD2) CSB-YP317026SMV
CSB-EP317026SMV
CSB-BP317026SMV
CSB-MP317026SMV
CSB-EP317026SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Lipoprotein signal peptidase (lspA), partial CSB-YP317027SMW1
CSB-EP317027SMW1
CSB-BP317027SMW1
CSB-MP317027SMW1
CSB-EP317027SMW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP317028SMV1
CSB-EP317028SMV1
CSB-BP317028SMV1
CSB-MP317028SMV1
CSB-EP317028SMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 S-ribosylhomocysteine lyase (luxS) CSB-YP317029SMW
CSB-EP317029SMW
CSB-BP317029SMW
CSB-MP317029SMW
CSB-EP317029SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Adapter protein mecA (mecA) CSB-YP317030SMW
CSB-EP317030SMW
CSB-BP317030SMW
CSB-MP317030SMW
CSB-EP317030SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP317031SMV
CSB-EP317031SMV
CSB-BP317031SMV
CSB-MP317031SMV
CSB-EP317031SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrA (msrA.2) CSB-YP317032SMV
CSB-EP317032SMV
CSB-BP317032SMV
CSB-MP317032SMV
CSB-EP317032SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP317033SMV
CSB-EP317033SMV
CSB-BP317033SMV
CSB-MP317033SMV
CSB-EP317033SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP317034SMW
CSB-EP317034SMW
CSB-BP317034SMW
CSB-MP317034SMW
CSB-EP317034SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vipera ammodytes ammodytes Factor X-activator 2 light chain 1 CSB-YP317035VFO
CSB-EP317035VFO
CSB-BP317035VFO
CSB-MP317035VFO
CSB-EP317035VFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus DNA-directed RNA polymerase subunit 2 (Ken-065), partial CSB-YP317036AEB
CSB-EP317036AEB
CSB-BP317036AEB
CSB-MP317036AEB
CSB-EP317036AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Potassium channel toxin alpha-KTx 13.4 CSB-YP317037TEV
CSB-EP317037TEV
CSB-BP317037TEV
CSB-MP317037TEV
CSB-EP317037TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Serine proteinase-like BMK-CBP CSB-YP317038MRE
CSB-EP317038MRE
CSB-BP317038MRE
CSB-MP317038MRE
CSB-EP317038MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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