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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanosarcina mazei Trk system potassium uptake protein trkA homolog 1 (trkA1) CSB-YP316210MFC
CSB-EP316210MFC
CSB-BP316210MFC
CSB-MP316210MFC
CSB-EP316210MFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Trk system potassium uptake protein trkA homolog 1 (trkA1) CSB-YP316211MSN
CSB-EP316211MSN
CSB-BP316211MSN
CSB-MP316211MSN
CSB-EP316211MSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 40S ribosomal protein S17-like (RPS17L) CSB-YP316212HU
CSB-EP316212HU
CSB-BP316212HU
CSB-MP316212HU
CSB-EP316212HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coiled-coil domain-containing protein 166 (CCDC166) CSB-YP316213HU
CSB-EP316213HU
CSB-BP316213HU
CSB-MP316213HU
CSB-EP316213HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB16 (vapB16) CSB-YP316214MVZ
CSB-EP316214MVZ
CSB-BP316214MVZ
CSB-MP316214MVZ
CSB-EP316214MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB48 (vapB48) CSB-YP316215MVZ
CSB-EP316215MVZ
CSB-BP316215MVZ
CSB-MP316215MVZ
CSB-EP316215MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative antitoxin VapB5 (vapB5), partial CSB-YP316216MRU1
CSB-EP316216MRU1
CSB-BP316216MRU1
CSB-MP316216MRU1
CSB-EP316216MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogen fixation nifHD region glnB-like protein 2 (glnBII) CSB-YP316217MEY
CSB-EP316217MEY
CSB-BP316217MEY
CSB-MP316217MEY
CSB-EP316217MEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogen fixation nifHD region glnB-like protein 2 (glnBII) CSB-YP316218MSB
CSB-EP316218MSB
CSB-BP316218MSB
CSB-MP316218MSB
CSB-EP316218MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP316219MSB
CSB-EP316219MSB
CSB-BP316219MSB
CSB-MP316219MSB
CSB-EP316219MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA repair and recombination protein radA (radA) CSB-YP316220MSB
CSB-EP316220MSB
CSB-BP316220MSB
CSB-MP316220MSB
CSB-EP316220MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Lysine--tRNA ligase (lysS), partial CSB-YP316221MSB
CSB-EP316221MSB
CSB-BP316221MSB
CSB-MP316221MSB
CSB-EP316221MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0212 protein MMP1509 (MMP1509) CSB-YP316222MSB
CSB-EP316222MSB
CSB-BP316222MSB
CSB-MP316222MSB
CSB-EP316222MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Antitoxin ParD3 (parD3) CSB-YP316223DQO
CSB-EP316223DQO
CSB-BP316223DQO
CSB-MP316223DQO
CSB-EP316223DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cadmium/zinc-transporting ATPase HMA3 (HMA3), partial CSB-YP316224DOA
CSB-EP316224DOA
CSB-BP316224DOA
CSB-MP316224DOA
CSB-EP316224DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Multidrug resistance protein EbrB (ebrB), partial CSB-YP316225BRJ1
CSB-EP316225BRJ1
CSB-BP316225BRJ1
CSB-MP316225BRJ1
CSB-EP316225BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein fis (fis) CSB-YP316226EZA
CSB-EP316226EZA
CSB-BP316226EZA
CSB-MP316226EZA
CSB-EP316226EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA repair and recombination protein radA (radA) CSB-YP316227ADR
CSB-EP316227ADR
CSB-BP316227ADR
CSB-MP316227ADR
CSB-EP316227ADR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coccidioides posadasii Heat-stable 19 kDa antigen (CSA) CSB-YP316228DUW
CSB-EP316228DUW
CSB-BP316228DUW
CSB-MP316228DUW
CSB-EP316228DUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coccidioides posadasii Wall-associated proteinase (CPC735_016940), partial CSB-YP316229DUW
CSB-EP316229DUW
CSB-BP316229DUW
CSB-MP316229DUW
CSB-EP316229DUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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