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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant African swine fever virus ERCC4 domain-containing protein EP364R (Mal-067) CSB-YP316675AYE
CSB-EP316675AYE
CSB-BP316675AYE
CSB-MP316675AYE
CSB-EP316675AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein K421R (Ken-064) CSB-YP316676AEB
CSB-EP316676AEB
CSB-BP316676AEB
CSB-MP316676AEB
CSB-EP316676AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein E423R (Pret-139) CSB-YP316677AYG
CSB-EP316677AYG
CSB-BP316677AYG
CSB-MP316677AYG
CSB-EP316677AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein DP63R (Mal-156) CSB-YP316678AYE
CSB-EP316678AYE
CSB-BP316678AYE
CSB-MP316678AYE
CSB-EP316678AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein C717R (Mal-072), partial CSB-YP316679AYE
CSB-EP316679AYE
CSB-BP316679AYE
CSB-MP316679AYE
CSB-EP316679AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein I73R (Pret-153) CSB-YP316680AYG
CSB-EP316680AYG
CSB-BP316680AYG
CSB-MP316680AYG
CSB-EP316680AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein L83L (War-004) CSB-YP316681AEI
CSB-EP316681AEI
CSB-BP316681AEI
CSB-MP316681AEI
CSB-EP316681AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized membrane protein KP93L (War-001), partial CSB-YP316682AEI1
CSB-EP316682AEI1
CSB-BP316682AEI1
CSB-MP316682AEI1
CSB-EP316682AEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein DP238L (Mal-154) CSB-YP316683AYE
CSB-EP316683AYE
CSB-BP316683AYE
CSB-MP316683AYE
CSB-EP316683AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana V-type proton ATPase subunit E3 (VHA-E3) CSB-YP316684DOA
CSB-EP316684DOA
CSB-BP316684DOA
CSB-MP316684DOA
CSB-EP316684DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA-directed RNA polymerase II subunit GRINL1A (POLR2M) CSB-YP316685HU
CSB-EP316685HU
CSB-BP316685HU
CSB-MP316685HU
CSB-EP316685HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Ribonucleoside-diphosphate reductase small chain (BaRF1) CSB-YP316686EEZ
CSB-EP316686EEZ
CSB-BP316686EEZ
CSB-MP316686EEZ
CSB-EP316686EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus achatinus Kappa-conotoxin-like Ac4.3b CSB-YP316687DVS
CSB-EP316687DVS
CSB-BP316687DVS
CSB-MP316687DVS
CSB-EP316687DVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus pyrrhocryptus Protease inhibitor G1 CSB-YP316688MGP
CSB-EP316688MGP
CSB-BP316688MGP
CSB-MP316688MGP
CSB-EP316688MGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus pyrrhocryptus Short neurotoxin D2A CSB-YP316689MGP
CSB-EP316689MGP
CSB-BP316689MGP
CSB-MP316689MGP
CSB-EP316689MGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus pyrrhocryptus Venom protein E2 CSB-YP316690MGP
CSB-EP316690MGP
CSB-BP316690MGP
CSB-MP316690MGP
CSB-EP316690MGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus durissus ruruima Phospholipase A2 Cdr-12 CSB-YP316691CSX
CSB-EP316691CSX
CSB-BP316691CSX
CSB-MP316691CSX
CSB-EP316691CSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus pyrrhocryptus Phospholipase A2 D5 CSB-YP316692MGP
CSB-EP316692MGP
CSB-BP316692MGP
CSB-MP316692MGP
CSB-EP316692MGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalitozoon hellem Polar tube protein 2 (PTP2) CSB-YP316693EKI
CSB-EP316693EKI
CSB-BP316693EKI
CSB-MP316693EKI
CSB-EP316693EKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 10 kDa chaperonin (groS) CSB-YP316694DQO
CSB-EP316694DQO
CSB-BP316694DQO
CSB-MP316694DQO
CSB-EP316694DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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