Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Diphthamide biosynthesis protein 3 (DPH3) CSB-YP317281CTL
CSB-EP317281CTL
CSB-BP317281CTL
CSB-MP317281CTL
CSB-EP317281CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA-dihydrouridine (47) synthase [NAD (P) (+)], partial CSB-YP317282CTK
CSB-EP317282CTK
CSB-BP317282CTK
CSB-MP317282CTK
CSB-EP317282CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Elongation factor G, mitochondrial (MEF1), partial CSB-YP317283CTK
CSB-EP317283CTK
CSB-BP317283CTK
CSB-MP317283CTK
CSB-EP317283CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Elongation factor Ts, mitochondrial (TSF1) CSB-YP317284CTL
CSB-EP317284CTL
CSB-BP317284CTL
CSB-MP317284CTL
CSB-EP317284CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Enhancer of polycomb-like protein 1 (EPL1), partial CSB-YP317285CTK
CSB-EP317285CTK
CSB-BP317285CTK
CSB-MP317285CTK
CSB-EP317285CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Exocyst complex component EXO84 (EXO84), partial CSB-YP317286CTK
CSB-EP317286CTK
CSB-BP317286CTK
CSB-MP317286CTK
CSB-EP317286CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondria fission 1 protein (FIS1), partial CSB-YP317287CTK
CSB-EP317287CTK
CSB-BP317287CTK
CSB-MP317287CTK
CSB-EP317287CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Anthranilate synthase component 2 (TRP1), partial CSB-YP317288CTL
CSB-EP317288CTL
CSB-BP317288CTL
CSB-MP317288CTL
CSB-EP317288CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Anthranilate synthase component 2 (TRP1), partial CSB-YP317289CTK
CSB-EP317289CTK
CSB-BP317289CTK
CSB-MP317289CTK
CSB-EP317289CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A (HTA1) CSB-YP317290CTL
CSB-EP317290CTL
CSB-BP317290CTL
CSB-MP317290CTL
CSB-EP317290CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1) CSB-YP317291CTL
CSB-EP317291CTL
CSB-BP317291CTL
CSB-MP317291CTL
CSB-EP317291CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1) CSB-YP317292CTK
CSB-EP317292CTK
CSB-BP317292CTK
CSB-MP317292CTK
CSB-EP317292CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Putative DNA helicase INO80 (INO80), partial CSB-YP317294CTK
CSB-EP317294CTK
CSB-BP317294CTK
CSB-MP317294CTK
CSB-EP317294CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Imidazoleglycerol-phosphate dehydratase (HIS3) CSB-YP317295CTL
CSB-EP317295CTL
CSB-BP317295CTL
CSB-MP317295CTL
CSB-EP317295CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Multiprotein-bridging factor 1 (MBF1) CSB-YP317296CTL
CSB-EP317296CTL
CSB-BP317296CTL
CSB-MP317296CTL
CSB-EP317296CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor ISY1 (ISY1) CSB-YP317297CTL
CSB-EP317297CTL
CSB-BP317297CTL
CSB-MP317297CTL
CSB-EP317297CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor ISY1 (ISY1) CSB-YP317298CTK
CSB-EP317298CTK
CSB-BP317298CTK
CSB-MP317298CTK
CSB-EP317298CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Branchpoint-bridging protein (BBP), partial CSB-YP317299CTL
CSB-EP317299CTL
CSB-BP317299CTL
CSB-MP317299CTL
CSB-EP317299CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent DNA helicase II subunit 1 (KU70), partial CSB-YP317300CTK
CSB-EP317300CTK
CSB-BP317300CTK
CSB-MP317300CTK
CSB-EP317300CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial distribution and morphology protein 10 (MDM10), partial CSB-YP317301CTK
CSB-EP317301CTK
CSB-BP317301CTK
CSB-MP317301CTK
CSB-EP317301CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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