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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gorilla gorilla gorilla Polyubiquitin-B (UBB) CSB-YP316736GGZ
CSB-EP316736GGZ
CSB-BP316736GGZ
CSB-MP316736GGZ
CSB-EP316736GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Polyubiquitin (UBI1) CSB-YP316737CZF
CSB-EP316737CZF
CSB-BP316737CZF
CSB-MP316737CZF
CSB-EP316737CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Polyubiquitin (ubi4) CSB-YP316738KBK
CSB-EP316738KBK
CSB-BP316738KBK
CSB-MP316738KBK
CSB-EP316738KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable F-box protein At4g22165 (At4g22165) CSB-YP316739DOA
CSB-EP316739DOA
CSB-BP316739DOA
CSB-MP316739DOA
CSB-EP316739DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Conotoxin pr6b CSB-YP316740COZ
CSB-EP316740COZ
CSB-BP316740COZ
CSB-MP316740COZ
CSB-EP316740COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus achatinus Conotoxin Ac3.1 CSB-YP316741DVS
CSB-EP316741DVS
CSB-BP316741DVS
CSB-MP316741DVS
CSB-EP316741DVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Polyubiquitin 11 (UBQ11) CSB-YP316742DOA
CSB-EP316742DOA
CSB-BP316742DOA
CSB-MP316742DOA
CSB-EP316742DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyriopagopus schmidti Tau-theraphotoxin-Hs1a CSB-YP316743DRU
CSB-BP316743DRU
CSB-MP316743DRU
CSB-EP316743DRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Neurotoxin Nv1-116.39.1 (v1g113166) CSB-YP316744NGO
CSB-EP316744NGO
CSB-BP316744NGO
CSB-MP316744NGO
CSB-EP316744NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chilobrachys jingzhao Jingzhaotoxin F5-21.66 CSB-YP316745DRU
CSB-EP316745DRU
CSB-BP316745DRU
CSB-MP316745DRU
CSB-EP316745DRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chilobrachys jingzhao Jingzhaotoxin F7-10.36 CSB-YP316746DRU
CSB-EP316746DRU
CSB-BP316746DRU
CSB-MP316746DRU
CSB-EP316746DRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum Hainantoxin F1-31.97 CSB-YP316747HUK
CSB-EP316747HUK
CSB-BP316747HUK
CSB-MP316747HUK
CSB-EP316747HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum Hainantoxin F8-17.06 CSB-YP316748HUK
CSB-EP316748HUK
CSB-BP316748HUK
CSB-MP316748HUK
CSB-EP316748HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa crabro Venom phospholipase A1 CSB-YP316749VBP
CSB-EP316749VBP
CSB-BP316749VBP
CSB-MP316749VBP
CSB-EP316749VBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ehrlichia chaffeensis Transcription termination factor Rho 2 (rho2) CSB-YP316750EJS
CSB-EP316750EJS
CSB-BP316750EJS
CSB-MP316750EJS
CSB-EP316750EJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative zinc finger protein 705D-like protein LOC100132396 CSB-YP316751HU
CSB-EP316751HU
CSB-BP316751HU
CSB-MP316751HU
CSB-EP316751HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Impages hectica Toxin hhe1a CSB-YP316752IAL
CSB-EP316752IAL
CSB-BP316752IAL
CSB-MP316752IAL
CSB-EP316752IAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Impages hectica Toxin hhe9 CSB-YP316753IAL
CSB-EP316753IAL
CSB-BP316753IAL
CSB-MP316753IAL
CSB-EP316753IAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Inclusion membrane protein A (incA), partial CSB-YP316754DSB1
CSB-EP316754DSB1
CSB-BP316754DSB1
CSB-MP316754DSB1
CSB-EP316754DSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris L-lactate dehydrogenase 2 (ldhB) CSB-YP316755LND
CSB-EP316755LND
CSB-BP316755LND
CSB-MP316755LND
CSB-EP316755LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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