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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D GDP-mannose transporter 2 (GMT2), partial CSB-YP316897CTL
CSB-EP316897CTL
CSB-BP316897CTL
CSB-MP316897CTL
CSB-EP316897CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRM61 CSB-YP316898CTL
CSB-EP316898CTL
CSB-BP316898CTL
CSB-MP316898CTL
CSB-EP316898CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ubiquitin-conjugating enzyme E2 2 (UBC2) CSB-YP316899CTL
CSB-EP316899CTL
CSB-BP316899CTL
CSB-MP316899CTL
CSB-EP316899CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UPF0390 protein CND04920 (CND04920) CSB-YP316900CTL
CSB-EP316900CTL
CSB-BP316900CTL
CSB-MP316900CTL
CSB-EP316900CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 18 (ATG18) CSB-YP316901CTK
CSB-EP316901CTK
CSB-BP316901CTK
CSB-MP316901CTK
CSB-EP316901CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP316902CTL
CSB-EP316902CTL
CSB-BP316902CTL
CSB-MP316902CTL
CSB-EP316902CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein HIR1 (HIR1), partial CSB-YP316903CTL
CSB-EP316903CTL
CSB-BP316903CTL
CSB-MP316903CTL
CSB-EP316903CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nuclear distribution protein PAC1 (PAC1) CSB-YP316904CTL
CSB-EP316904CTL
CSB-BP316904CTL
CSB-MP316904CTL
CSB-EP316904CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP316905CTL
CSB-EP316905CTL
CSB-BP316905CTL
CSB-MP316905CTL
CSB-EP316905CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D WD repeat-containing protein CNI03070 (CNI03070), partial CSB-YP316906CTL
CSB-EP316906CTL
CSB-BP316906CTL
CSB-MP316906CTL
CSB-EP316906CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein GET1 (GET1), partial CSB-YP316907CTK1
CSB-EP316907CTK1
CSB-BP316907CTK1
CSB-MP316907CTK1
CSB-EP316907CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D pH-response transcription factor pacC/RIM101 (RIM101), partial CSB-YP316908CTL
CSB-EP316908CTL
CSB-BP316908CTL
CSB-MP316908CTL
CSB-EP316908CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Palmitoyltransferase PFA4 (PFA4), partial CSB-YP316910CTK1
CSB-EP316910CTK1
CSB-BP316910CTK1
CSB-MP316910CTK1
CSB-EP316910CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SUS1 (SUS1) CSB-YP316911CTK
CSB-EP316911CTK
CSB-BP316911CTK
CSB-MP316911CTK
CSB-EP316911CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Serine/threonine-protein kinase SSN3 (SSN3) CSB-YP316912CTK
CSB-EP316912CTK
CSB-BP316912CTK
CSB-MP316912CTK
CSB-EP316912CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (guanine-N (7)-)-methyltransferase CSB-YP316913CTK
CSB-EP316913CTK
CSB-BP316913CTK
CSB-MP316913CTK
CSB-EP316913CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe UPF0494 membrane protein C1348.01 (SPBC1348.01), partial CSB-YP316914SXV1
CSB-EP316914SXV1
CSB-BP316914SXV1
CSB-MP316914SXV1
CSB-EP316914SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Convulxin subunit beta homolog, partial CSB-YP316915DYC
CSB-EP316915DYC
CSB-BP316915DYC
CSB-MP316915DYC
CSB-EP316915DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Snake venom serine protease homolog CSB-YP316916DYC
CSB-EP316916DYC
CSB-BP316916DYC
CSB-MP316916DYC
CSB-EP316916DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa (3S,6E)-nerolidol synthase 1, partial CSB-YP316917FEZ
CSB-EP316917FEZ
CSB-BP316917FEZ
CSB-MP316917FEZ
CSB-EP316917FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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