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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella typhimurium Oxygen-regulated invasion protein OrgB (orgB) CSB-YP317706SXB
CSB-EP317706SXB
CSB-BP317706SXB
CSB-MP317706SXB
CSB-EP317706SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Trehalose-phosphate phosphatase (otsB) CSB-YP317707SXB
CSB-EP317707SXB
CSB-BP317707SXB
CSB-MP317707SXB
CSB-EP317707SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae Putative glutathione-dependent formaldehyde-activating enzyme (AO090701000508-B) CSB-YP317708DPA
CSB-EP317708DPA
CSB-BP317708DPA
CSB-MP317708DPA
CSB-EP317708DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable antitoxin MazE1 (mazE1) CSB-YP317709MVZ
CSB-EP317709MVZ
CSB-BP317709MVZ
CSB-MP317709MVZ
CSB-EP317709MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin MazF7 (mazF7) CSB-YP317710MVZ
CSB-EP317710MVZ
CSB-BP317710MVZ
CSB-MP317710MVZ
CSB-EP317710MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease clavin (cla) CSB-YP317711APM
CSB-EP317711APM
CSB-BP317711APM
CSB-MP317711APM
CSB-EP317711APM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (lig), partial CSB-YP317712PXQ
CSB-EP317712PXQ
CSB-BP317712PXQ
CSB-MP317712PXQ
CSB-EP317712PXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi DNA ligase (lig), partial CSB-YP317713FHV
CSB-EP317713FHV
CSB-BP317713FHV
CSB-MP317713FHV
CSB-EP317713FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BFR2 (BFR2) CSB-YP317714CTL
CSB-EP317714CTL
CSB-BP317714CTL
CSB-MP317714CTL
CSB-EP317714CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Acetate non-utilizing protein 9, mitochondrial (ACN9) CSB-YP317715CTK
CSB-EP317715CTK
CSB-BP317715CTK
CSB-MP317715CTK
CSB-EP317715CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-N-acetylglucosamine transferase subunit ALG14 (ALG14), partial CSB-YP317716CTL
CSB-EP317716CTL
CSB-BP317716CTL
CSB-MP317716CTL
CSB-EP317716CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-N-acetylglucosamine transferase subunit ALG14 (ALG14), partial CSB-YP317717CTK
CSB-EP317717CTK
CSB-BP317717CTK
CSB-MP317717CTK
CSB-EP317717CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP317718CTL
CSB-EP317718CTL
CSB-BP317718CTL
CSB-MP317718CTL
CSB-EP317718CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pentafunctional AROM polypeptide (CNBB3730), partial CSB-YP317719CTK
CSB-EP317719CTK
CSB-BP317719CTK
CSB-MP317719CTK
CSB-EP317719CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone chaperone ASF1 (ASF1) CSB-YP317720CTK
CSB-EP317720CTK
CSB-BP317720CTK
CSB-MP317720CTK
CSB-EP317720CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 22 (ATG22), partial CSB-YP317721CTL
CSB-EP317721CTL
CSB-BP317721CTL
CSB-MP317721CTL
CSB-EP317721CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 22 (ATG22), partial CSB-YP317722CTK
CSB-EP317722CTK
CSB-BP317722CTK
CSB-MP317722CTK
CSB-EP317722CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BTN1 (BTN1), partial CSB-YP317723CTL
CSB-EP317723CTL
CSB-BP317723CTL
CSB-MP317723CTL
CSB-EP317723CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar protein-sorting protein BRO1 (BRO1), partial CSB-YP317724CTK
CSB-EP317724CTK
CSB-BP317724CTK
CSB-MP317724CTK
CSB-EP317724CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D pH-response regulator protein palC (CNBE3350) CSB-YP317725CTK
CSB-EP317725CTK
CSB-BP317725CTK
CSB-MP317725CTK
CSB-EP317725CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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