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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Sterol 3-beta-glucosyltransferase (ATG26), partial CSB-YP316836CTL
CSB-EP316836CTL
CSB-BP316836CTL
CSB-MP316836CTL
CSB-EP316836CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Guanine nucleotide-binding protein subunit alpha (GPA1) CSB-YP316837CTL
CSB-EP316837CTL
CSB-BP316837CTL
CSB-MP316837CTL
CSB-EP316837CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2B (HTB1) CSB-YP316838CTK
CSB-EP316838CTK
CSB-BP316838CTK
CSB-MP316838CTK
CSB-EP316838CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Putative DNA helicase INO80 (INO80), partial CSB-YP316840CTL
CSB-EP316840CTL
CSB-BP316840CTL
CSB-MP316840CTL
CSB-EP316840CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mannose-1-phosphate guanyltransferase (MPG1) CSB-YP316841CTK
CSB-EP316841CTK
CSB-BP316841CTK
CSB-MP316841CTK
CSB-EP316841CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar membrane-associated protein IML1 (IML1), partial CSB-YP316842CTL
CSB-EP316842CTL
CSB-BP316842CTL
CSB-MP316842CTL
CSB-EP316842CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Transcription factor IWS1 (IWS1) CSB-YP316843CTK
CSB-EP316843CTK
CSB-BP316843CTK
CSB-MP316843CTK
CSB-EP316843CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D JmjC domain-containing histone demethylation protein 1 (JHD1), partial CSB-YP316844CTK
CSB-EP316844CTK
CSB-BP316844CTK
CSB-MP316844CTK
CSB-EP316844CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 8 (ATG8) CSB-YP316845CTL
CSB-EP316845CTL
CSB-BP316845CTL
CSB-MP316845CTL
CSB-EP316845CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Lipoyl synthase, mitochondrial (CNBI2500) CSB-YP316846CTK
CSB-EP316846CTK
CSB-BP316846CTK
CSB-MP316846CTK
CSB-EP316846CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial distribution and morphology protein 12 (MDM12) CSB-YP316847CTL
CSB-EP316847CTL
CSB-BP316847CTL
CSB-MP316847CTL
CSB-EP316847CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Chromatin modification-related protein EAF3 (EAF3) CSB-YP316848CTK
CSB-EP316848CTK
CSB-BP316848CTK
CSB-MP316848CTK
CSB-EP316848CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CEF1 (CEF1), partial CSB-YP316849CTK
CSB-EP316849CTK
CSB-BP316849CTK
CSB-MP316849CTK
CSB-EP316849CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP316850CTL
CSB-EP316850CTL
CSB-BP316850CTL
CSB-MP316850CTL
CSB-EP316850CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable NADPH reductase TAH18 (TAH18), partial CSB-YP316851CTK
CSB-EP316851CTK
CSB-BP316851CTK
CSB-MP316851CTK
CSB-EP316851CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable kinetochore protein NDC80 (NDC80), partial CSB-YP316852CTL
CSB-EP316852CTL
CSB-BP316852CTL
CSB-MP316852CTL
CSB-EP316852CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Glycylpeptide N-tetradecanoyltransferase (CNBN0070) CSB-YP316853CTK
CSB-EP316853CTK
CSB-BP316853CTK
CSB-MP316853CTK
CSB-EP316853CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nuclear protein localization protein 4 (NPL4), partial CSB-YP316854CTL
CSB-EP316854CTL
CSB-BP316854CTL
CSB-MP316854CTL
CSB-EP316854CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-galactose transporter homolog 1 (HUT1-A), partial CSB-YP316855CTK
CSB-EP316855CTK
CSB-BP316855CTK
CSB-MP316855CTK
CSB-EP316855CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Lysophospholipase NTE1 (NTE1), partial CSB-YP316856CTL
CSB-EP316856CTL
CSB-BP316856CTL
CSB-MP316856CTL
CSB-EP316856CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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