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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Apocytochrome f (petA), partial CSB-YP317039SSC1
CSB-EP317039SSC1
CSB-BP317039SSC1
CSB-MP317039SSC1
CSB-EP317039SSC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiocillin GE37468 (getA) CSB-YP317040FOM
CSB-EP317040FOM
CSB-BP317040FOM
CSB-MP317040FOM
CSB-EP317040FOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g19025 (At5g19025), partial CSB-YP317041DOA1
CSB-EP317041DOA1
CSB-BP317041DOA1
CSB-MP317041DOA1
CSB-EP317041DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda colubrina Long neurotoxin 1 CSB-YP317042LCZ
CSB-EP317042LCZ
CSB-BP317042LCZ
CSB-MP317042LCZ
CSB-EP317042LCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Alpha-conotoxin PrXA CSB-YP317043COZ
CSB-EP317043COZ
CSB-BP317043COZ
CSB-MP317043COZ
CSB-EP317043COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana picturata Brevinin-2PTe CSB-YP317044RBD
CSB-EP317044RBD
CSB-BP317044RBD
CSB-MP317044RBD
CSB-EP317044RBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus pulicarius Alpha-conotoxin-like PuSG1.1 CSB-YP317045DWG
CSB-EP317045DWG
CSB-BP317045DWG
CSB-MP317045DWG
CSB-EP317045DWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus achatinus Delta-conotoxin-like Ac6.1 CSB-YP317046DVS
CSB-EP317046DVS
CSB-BP317046DVS
CSB-MP317046DVS
CSB-EP317046DVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus achatinus Omega-conotoxin-like Ac6.5 CSB-YP317047DVS
CSB-EP317047DVS
CSB-BP317047DVS
CSB-MP317047DVS
CSB-EP317047DVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Peptide 7629 CSB-YP317048TEV
CSB-EP317048TEV
CSB-BP317048TEV
CSB-MP317048TEV
CSB-EP317048TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Zinc metalloproteinase CSB-YP317049TEV
CSB-EP317049TEV
CSB-BP317049TEV
CSB-MP317049TEV
CSB-EP317049TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bertholletia excelsa 2S sulfur-rich seed storage protein 2 (BE2S2), partial CSB-YP317050BGJ
CSB-EP317050BGJ
CSB-BP317050BGJ
CSB-MP317050BGJ
CSB-EP317050BGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Fimbrillin matB homolog (matB) CSB-YP317051EOP
CSB-EP317051EOP
CSB-BP317051EOP
CSB-MP317051EOP
CSB-EP317051EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus leopardus Conotoxin Leo-T2 CSB-YP317052CXD
CSB-EP317052CXD
CSB-BP317052CXD
CSB-MP317052CXD
CSB-EP317052CXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Overexpressed in colon carcinoma 1 protein homolog CSB-YP317053MO
CSB-EP317053MO
CSB-BP317053MO
CSB-MP317053MO
CSB-EP317053MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eumenes rubronotatus Eumenitin CSB-YP317054EIB
CSB-EP317054EIB
CSB-BP317054EIB
CSB-MP317054EIB
CSB-EP317054EIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Chaperone protein DnaK (dnaK), partial CSB-YP317055EYA
CSB-EP317055EYA
CSB-BP317055EYA
CSB-MP317055EYA
CSB-EP317055EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At1g21580 (At1g21580), partial CSB-YP317056DOA
CSB-EP317056DOA
CSB-BP317056DOA
CSB-MP317056DOA
CSB-EP317056DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata 1,3-beta-glucanosyltransferase gel3 (gel3) CSB-YP317057NGS
CSB-EP317057NGS
CSB-BP317057NGS
CSB-MP317057NGS
CSB-EP317057NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable methyltransferase EP424R (War-065) CSB-YP317058AEI
CSB-EP317058AEI
CSB-BP317058AEI
CSB-MP317058AEI
CSB-EP317058AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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