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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Canine distemper virus Fusion glycoprotein F0 (F), partial CSB-YP318261CCQ
CSB-BP318261CCQ
CSB-MP318261CCQ
CSB-EP318261CCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Fusion glycoprotein F0 (F), partial CSB-YP318262SEX
CSB-EP318262SEX
CSB-BP318262SEX
CSB-MP318262SEX
CSB-EP318262SEX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Vanillate O-demethylase oxidoreductase (vanB) CSB-YP318263PVI
CSB-EP318263PVI
CSB-BP318263PVI
CSB-MP318263PVI
CSB-EP318263PVI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP318264ICM
CSB-EP318264ICM
CSB-BP318264ICM
CSB-MP318264ICM
CSB-EP318264ICM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP318265ICK
CSB-EP318265ICK
CSB-BP318265ICK
CSB-MP318265ICK
CSB-EP318265ICK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP318266ICJ
CSB-EP318266ICJ
CSB-BP318266ICJ
CSB-MP318266ICJ
CSB-EP318266ICJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Non-structural protein 1 (NS) CSB-YP318267IJJ
CSB-EP318267IJJ
CSB-BP318267IJJ
CSB-MP318267IJJ
CSB-EP318267IJJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Non-structural protein 1 (NS) CSB-YP318268IJT
CSB-EP318268IJT
CSB-BP318268IJT
CSB-MP318268IJT
CSB-EP318268IJT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Integrin beta-1-A (itgb1-a), partial CSB-YP318269XBE
CSB-EP318269XBE
CSB-BP318269XBE
CSB-MP318269XBE
CSB-EP318269XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Vanillate O-demethylase oxygenase subunit (vanA) CSB-YP318270PVI
CSB-EP318270PVI
CSB-BP318270PVI
CSB-MP318270PVI
CSB-EP318270PVI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen fixation protein AnfA (anfA), partial CSB-YP318271DPX
CSB-EP318271DPX
CSB-BP318271DPX
CSB-MP318271DPX
CSB-EP318271DPX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Uptake hydrogenase large subunit (hupB), partial CSB-YP318272BVW
CSB-EP318272BVW
CSB-BP318272BVW
CSB-MP318272BVW
CSB-EP318272BVW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial CSB-YP318273ARW
CSB-BP318273ARW
CSB-MP318273ARW
CSB-EP318273ARW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA-entry nuclease (nucA), partial CSB-YP318274BRJ
CSB-EP318274BRJ
CSB-BP318274BRJ
CSB-MP318274BRJ
CSB-EP318274BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae High-affinity potassium transport protein (TRK1), partial CSB-YP318275SVG
CSB-EP318275SVG
CSB-BP318275SVG
CSB-MP318275SVG
CSB-EP318275SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas oleovorans Alkane 1-monooxygenase (alkB), partial CSB-YP318276PUQ1
CSB-EP318276PUQ1
CSB-BP318276PUQ1
CSB-MP318276PUQ1
CSB-EP318276PUQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Insulin-1 (ins-a) CSB-YP318277XBE
CSB-EP318277XBE
CSB-BP318277XBE
CSB-MP318277XBE
CSB-EP318277XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin, cytoplasmic CSB-YP318278OGC
CSB-EP318278OGC
CSB-BP318278OGC
CSB-MP318278OGC
CSB-EP318278OGC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem Q (B) protein, partial CSB-YP318279DZX1
CSB-EP318279DZX1
CSB-BP318279DZX1
CSB-MP318279DZX1
CSB-EP318279DZX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Replicase polyprotein 1ab (rep) CSB-YP318280ARV
CSB-EP318280ARV
CSB-EP318280ARV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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