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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Spinacia oleracea Oxygen-evolving enhancer protein 3, chloroplastic (PSBQ) CSB-YP318943FKI
CSB-EP318943FKI
CSB-BP318943FKI
CSB-MP318943FKI
CSB-EP318943FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein X92, partial CSB-YP318944TQV
CSB-EP318944TQV
CSB-BP318944TQV
CSB-MP318944TQV
CSB-EP318944TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lathyrus ochrus Mannose/glucose-specific lectin alpha 2 chain CSB-YP318945LCV
CSB-EP318945LCV
CSB-BP318945LCV
CSB-MP318945LCV
CSB-EP318945LCV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glucose 1-dehydrogenase (gdh) CSB-YP318946BRJ
CSB-EP318946BRJ
CSB-BP318946BRJ
CSB-MP318946BRJ
CSB-EP318946BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP318947EMX
CSB-EP318947EMX
CSB-BP318947EMX
CSB-MP318947EMX
CSB-EP318947EMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Glandular kallikrein, prostatic CSB-YP318948GU
CSB-EP318948GU
CSB-BP318948GU
CSB-MP318948GU
CSB-EP318948GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna gibba Chlorophyll a-b binding protein of LHCII type I, chloroplastic, partial CSB-YP318949LDU1
CSB-EP318949LDU1
CSB-BP318949LDU1
CSB-MP318949LDU1
CSB-EP318949LDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia acetabulum Period clock protein CSB-YP318950AAV
CSB-EP318950AAV
CSB-BP318950AAV
CSB-MP318950AAV
CSB-EP318950AAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mating pheromone Er-10 (MAT10) CSB-YP318951EIL
CSB-EP318951EIL
CSB-BP318951EIL
CSB-MP318951EIL
CSB-EP318951EIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Oxygen-evolving enhancer protein 1, chloroplastic (PSBO) CSB-YP318952FKI
CSB-EP318952FKI
CSB-BP318952FKI
CSB-MP318952FKI
CSB-EP318952FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III restriction-modification system EcoP15I enzyme mod (mod) CSB-YP318953ENL
CSB-EP318953ENL
CSB-BP318953ENL
CSB-MP318953ENL
CSB-EP318953ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster cAMP-dependent protein kinase catalytic subunit (Pka-C1) CSB-YP318954DLU
CSB-BP318954DLU
CSB-MP318954DLU
CSB-EP318954DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Copper resistance protein B (copB) CSB-YP318955PWG
CSB-EP318955PWG
CSB-BP318955PWG
CSB-MP318955PWG
CSB-EP318955PWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Pheromone alpha factor receptor (STE2), partial CSB-YP318956LMA1
CSB-EP318956LMA1
CSB-BP318956LMA1
CSB-MP318956LMA1
CSB-EP318956LMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. ATP synthase subunit c (atpE), partial CSB-YP318957NHR1
CSB-EP318957NHR1
CSB-BP318957NHR1
CSB-MP318957NHR1
CSB-EP318957NHR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoprotein (N) CSB-YP318958AFK
CSB-EP318958AFK
CSB-BP318958AFK
CSB-MP318958AFK
CSB-EP318958AFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anarhichas lupus Type-3 ice-structuring protein 1.5 CSB-YP318959AJS
CSB-EP318959AJS
CSB-BP318959AJS
CSB-MP318959AJS
CSB-EP318959AJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 1 Outer capsid protein VP2 (S2), partial CSB-YP318960BHN
CSB-EP318960BHN
CSB-BP318960BHN
CSB-MP318960BHN
CSB-EP318960BHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 1 Outer capsid protein VP5 (S6), partial CSB-YP318961BHN
CSB-EP318961BHN
CSB-BP318961BHN
CSB-MP318961BHN
CSB-EP318961BHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA) CSB-YP318962IIS
CSB-EP318962IIS
CSB-BP318962IIS
CSB-MP318962IIS
CSB-EP318962IIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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