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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Latrodectus hasseltii Alpha-latroinsectotoxin-Lh1a CSB-YP319145LNU
CSB-EP319145LNU
CSB-BP319145LNU
CSB-MP319145LNU
CSB-EP319145LNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptelytrops albolabris Thrombin-like enzyme albolabrase CSB-YP319146DYI
CSB-EP319146DYI
CSB-BP319146DYI
CSB-MP319146DYI
CSB-EP319146DYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 23.6 kDa protein CSB-YP319147BRN
CSB-EP319147BRN
CSB-BP319147BRN
CSB-MP319147BRN
CSB-EP319147BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein psiB (psiB) CSB-YP319148ENV
CSB-EP319148ENV
CSB-BP319148ENV
CSB-MP319148ENV
CSB-EP319148ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus thunbergii Chlorophyll a-b binding protein type I, chloroplastic, partial CSB-YP319149EVZ1
CSB-EP319149EVZ1
CSB-BP319149EVZ1
CSB-MP319149EVZ1
CSB-EP319149EVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus thunbergii Ribulose bisphosphate carboxylase small chain, chloroplastic (RBCS) CSB-YP319150EVZ
CSB-EP319150EVZ
CSB-BP319150EVZ
CSB-MP319150EVZ
CSB-EP319150EVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphenodon punctatus Hemoglobin subunit beta-2 (HBB2) CSB-YP319151SJJ
CSB-EP319151SJJ
CSB-BP319151SJJ
CSB-MP319151SJJ
CSB-EP319151SJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Regulatory protein brlA (brlA) CSB-YP319152EKF
CSB-EP319152EKF
CSB-BP319152EKF
CSB-MP319152EKF
CSB-EP319152EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP319153SVG
CSB-EP319153SVG
CSB-BP319153SVG
CSB-MP319153SVG
CSB-EP319153SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Probable sulfate/thiosulfate import ATP-binding protein cysA (cysA) CSB-YP319154MCB
CSB-EP319154MCB
CSB-BP319154MCB
CSB-MP319154MCB
CSB-EP319154MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Esterase-5 (Est-5) CSB-YP319155DLY
CSB-EP319155DLY
CSB-BP319155DLY
CSB-MP319155DLY
CSB-EP319155DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysis protein for colicin E8 (lys) CSB-YP319156ENL
CSB-EP319156ENL
CSB-BP319156ENL
CSB-MP319156ENL
CSB-EP319156ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Fibritin (wac) CSB-YP319157EDZ
CSB-BP319157EDZ
CSB-MP319157EDZ
CSB-EP319157EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Signal recognition particle receptor FtsY (ftsY) CSB-YP319158ENV
CSB-EP319158ENV
CSB-BP319158ENV
CSB-MP319158ENV
CSB-EP319158ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 30S ribosomal protein S5 (rpsE) CSB-YP319159MLJ
CSB-EP319159MLJ
CSB-BP319159MLJ
CSB-MP319159MLJ
CSB-EP319159MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 50S ribosomal protein L14 (rplN) CSB-YP319160MLJ
CSB-EP319160MLJ
CSB-BP319160MLJ
CSB-MP319160MLJ
CSB-EP319160MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 50S ribosomal protein L29 (rpmC) CSB-YP319161MLJ
CSB-EP319161MLJ
CSB-BP319161MLJ
CSB-MP319161MLJ
CSB-EP319161MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acidic proline-rich protein PRP25 CSB-YP319162RA
CSB-EP319162RA
CSB-BP319162RA
CSB-MP319162RA
CSB-EP319162RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opacity-related protein POPM3 (opr) CSB-YP319163NGI
CSB-EP319163NGI
CSB-BP319163NGI
CSB-MP319163NGI
CSB-EP319163NGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Glutaminase-asparaginase (ansB) CSB-YP319164PVE
CSB-EP319164PVE
CSB-BP319164PVE
CSB-MP319164PVE
CSB-EP319164PVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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