Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial intermediate peptidase 2 (OCT2), partial CSB-YP317767CTK
CSB-EP317767CTK
CSB-BP317767CTK
CSB-MP317767CTK
CSB-EP317767CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Adenylosuccinate synthetase (CNBC2420) CSB-YP317768CTK
CSB-EP317768CTK
CSB-BP317768CTK
CSB-MP317768CTK
CSB-EP317768CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 60S ribosomal protein L44 (RPL44) CSB-YP317770CTL
CSB-EP317770CTL
CSB-BP317770CTL
CSB-MP317770CTL
CSB-EP317770CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 40S ribosomal protein S1 (RPS1) CSB-YP317771CTL
CSB-EP317771CTL
CSB-BP317771CTL
CSB-MP317771CTL
CSB-EP317771CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribosome biogenesis protein NSA2 (NSA2) CSB-YP317772CTK
CSB-EP317772CTK
CSB-BP317772CTK
CSB-MP317772CTK
CSB-EP317772CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CWC24 (CWC24) CSB-YP317773CTK
CSB-EP317773CTK
CSB-BP317773CTK
CSB-MP317773CTK
CSB-EP317773CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Arginine N-methyltransferase 2 (RMT2) CSB-YP317774CTL
CSB-EP317774CTL
CSB-BP317774CTL
CSB-MP317774CTL
CSB-EP317774CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP2-A (DBP2), partial CSB-YP317775CTL
CSB-EP317775CTL
CSB-BP317775CTL
CSB-MP317775CTL
CSB-EP317775CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP4 (DBP4), partial CSB-YP317776CTL
CSB-EP317776CTL
CSB-BP317776CTL
CSB-MP317776CTL
CSB-EP317776CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase MAK5 (MAK5), partial CSB-YP317777CTK
CSB-EP317777CTK
CSB-BP317777CTK
CSB-MP317777CTK
CSB-EP317777CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase SUB2 (SUB2) CSB-YP317778CTL
CSB-EP317778CTL
CSB-BP317778CTL
CSB-MP317778CTL
CSB-EP317778CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent rRNA helicase RRP3 (RRP3) CSB-YP317779CTK
CSB-EP317779CTK
CSB-BP317779CTK
CSB-MP317779CTK
CSB-EP317779CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP10 (DBP10), partial CSB-YP317780CTL
CSB-EP317780CTL
CSB-BP317780CTL
CSB-MP317780CTL
CSB-EP317780CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase MRH4, mitochondrial (MRH4), partial CSB-YP317781CTL
CSB-EP317781CTL
CSB-BP317781CTL
CSB-MP317781CTL
CSB-EP317781CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Multiple RNA-binding domain-containing protein 1 (MRD1), partial CSB-YP317782CTL
CSB-EP317782CTL
CSB-BP317782CTL
CSB-MP317782CTL
CSB-EP317782CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D rRNA biogenesis protein RRP36 (RRP36) CSB-YP317783CTK
CSB-EP317783CTK
CSB-BP317783CTK
CSB-MP317783CTK
CSB-EP317783CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D RuvB-like helicase 1 (RVB1) CSB-YP317784CTL
CSB-EP317784CTL
CSB-BP317784CTL
CSB-MP317784CTL
CSB-EP317784CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 3-ketodihydrosphingosine reductase TSC10 (TSC10), partial CSB-YP317785CTK1
CSB-EP317785CTK1
CSB-BP317785CTK1
CSB-MP317785CTK1
CSB-EP317785CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein transport protein SEC23 (SEC23), partial CSB-YP317786CTL
CSB-EP317786CTL
CSB-BP317786CTL
CSB-MP317786CTL
CSB-EP317786CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Phosphatidylinositol transfer protein SFH5 (SFH5) CSB-YP317787CTL
CSB-EP317787CTL
CSB-BP317787CTL
CSB-MP317787CTL
CSB-EP317787CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>