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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Eleginus gracilis Ice-structuring glycoprotein 7R CSB-YP319347EBG
CSB-EP319347EBG
CSB-BP319347EBG
CSB-MP319347EBG
CSB-EP319347EBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein KAR1 (KAR1) CSB-YP319348SVG
CSB-EP319348SVG
CSB-BP319348SVG
CSB-MP319348SVG
CSB-EP319348SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Sex pheromone cAM373 CSB-YP319349EEF
CSB-EP319349EEF
CSB-BP319349EEF
CSB-MP319349EEF
CSB-EP319349EEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial CSB-YP319350SVG
CSB-BP319350SVG
CSB-MP319350SVG
CSB-EP319350SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium L-threonine dehydratase catabolic TdcB (tdcB) CSB-YP319351SXB
CSB-EP319351SXB
CSB-BP319351SXB
CSB-MP319351SXB
CSB-EP319351SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrullus lanatus Trypsin inhibitor 1 CSB-YP319352CKU
CSB-EP319352CKU
CSB-BP319352CKU
CSB-MP319352CKU
CSB-EP319352CKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Phosphoglycerate kinase (pgkA) CSB-YP319353EKF
CSB-EP319353EKF
CSB-BP319353EKF
CSB-MP319353EKF
CSB-EP319353EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Methane monooxygenase component A gamma chain (mmoZ) CSB-YP319354MFM
CSB-EP319354MFM
CSB-BP319354MFM
CSB-MP319354MFM
CSB-EP319354MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Larval serum protein 1 beta chain (Lsp1beta), partial CSB-YP319355DLU
CSB-EP319355DLU
CSB-BP319355DLU
CSB-MP319355DLU
CSB-EP319355DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 6-phosphogluconate dehydrogenase, decarboxylating (gntZ) CSB-YP319356BRJ
CSB-EP319356BRJ
CSB-BP319356BRJ
CSB-MP319356BRJ
CSB-EP319356BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Polysialoglycoprotein CSB-YP319358OEI
CSB-EP319358OEI
CSB-BP319358OEI
CSB-MP319358OEI
CSB-EP319358OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoribosylamine--glycine ligase (purD) CSB-YP319359BRJ
CSB-EP319359BRJ
CSB-BP319359BRJ
CSB-MP319359BRJ
CSB-EP319359BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP319360BRJ
CSB-EP319360BRJ
CSB-BP319360BRJ
CSB-MP319360BRJ
CSB-EP319360BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer membrane usher protein fanD (fanD) CSB-YP319361ENL
CSB-EP319361ENL
CSB-BP319361ENL
CSB-MP319361ENL
CSB-EP319361ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pilin (traA), partial CSB-YP319362SWW1
CSB-EP319362SWW1
CSB-BP319362SWW1
CSB-MP319362SWW1
CSB-EP319362SWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Axis axis Lysozyme C-1/C-2 CSB-YP319363ATP
CSB-EP319363ATP
CSB-BP319363ATP
CSB-MP319363ATP
CSB-EP319363ATP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Xylose isomerase (xylA) CSB-YP319364AOO
CSB-EP319364AOO
CSB-BP319364AOO
CSB-MP319364AOO
CSB-EP319364AOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhigophila dearborni Ice-structuring protein RD2 CSB-YP319365RCP
CSB-EP319365RCP
CSB-BP319365RCP
CSB-MP319365RCP
CSB-EP319365RCP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Cytochrome b6 (petB), partial CSB-YP319367NEK1
CSB-EP319367NEK1
CSB-BP319367NEK1
CSB-MP319367NEK1
CSB-EP319367NEK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP319368OFG
CSB-EP319368OFG
CSB-BP319368OFG
CSB-MP319368OFG
CSB-EP319368OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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