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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M3 UPF0337 protein SPs1721 (SPs1721) CSB-YP317975SMW
CSB-EP317975SMW
CSB-BP317975SMW
CSB-MP317975SMW
CSB-EP317975SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0342 protein SpyM3_0545 (SpyM3_0545) CSB-YP317976SMV
CSB-EP317976SMV
CSB-BP317976SMV
CSB-MP317976SMV
CSB-EP317976SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0371 protein SPs0839 (SPs0839) CSB-YP317977SMW
CSB-EP317977SMW
CSB-BP317977SMW
CSB-MP317977SMW
CSB-EP317977SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0473 protein SPs1794 (SPs1794) CSB-YP317978SMW
CSB-EP317978SMW
CSB-BP317978SMW
CSB-MP317978SMW
CSB-EP317978SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerS (xerS) CSB-YP317979SMV
CSB-EP317979SMV
CSB-BP317979SMV
CSB-MP317979SMV
CSB-EP317979SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Selaginella moellendorffii SCA7 domain-containing protein SELMODRAFT_431321 (SELMODRAFT_431321) CSB-YP317980SYF
CSB-EP317980SYF
CSB-BP317980SYF
CSB-MP317980SYF
CSB-EP317980SYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative regulatory protein PrgT (prgT) CSB-YP317981ELW
CSB-EP317981ELW
CSB-BP317981ELW
CSB-MP317981ELW
CSB-EP317981ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RING finger protein 224 (RNF224) CSB-YP317982HU
CSB-EP317982HU
CSB-BP317982HU
CSB-MP317982HU
CSB-EP317982HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adiantum capillus-veneris CASP-like protein 1, partial CSB-YP317983AXO1
CSB-EP317983AXO1
CSB-BP317983AXO1
CSB-MP317983AXO1
CSB-EP317983AXO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative 12-oxophytodienoate reductase-like protein 2A (At1g17990) CSB-YP317984DOA
CSB-EP317984DOA
CSB-BP317984DOA
CSB-MP317984DOA
CSB-EP317984DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Lipoprotein p33 (MYPE6800) CSB-YP317985MLU
CSB-EP317985MLU
CSB-BP317985MLU
CSB-MP317985MLU
CSB-EP317985MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 30S ribosomal protein S3 (rpsC) CSB-YP317986MEN
CSB-EP317986MEN
CSB-BP317986MEN
CSB-MP317986MEN
CSB-EP317986MEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaejovis mexicanus smithi Potassium channel toxin alpha-KTx 21.1 CSB-YP317987VAK
CSB-EP317987VAK
CSB-BP317987VAK
CSB-EP317987VAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus petersii Potassium channel toxin kappa-KTx 3.3 CSB-YP317988HGR
CSB-EP317988HGR
CSB-BP317988HGR
CSB-MP317988HGR
CSB-EP317988HGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-853 CSB-YP317989HUK
CSB-EP317989HUK
CSB-BP317989HUK
CSB-MP317989HUK
CSB-EP317989HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti HWTX-XI-IS2 CSB-YP317990HUL
CSB-EP317990HUL
CSB-BP317990HUL
CSB-MP317990HUL
CSB-EP317990HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti HWTX-XI-IS22 CSB-YP317991HUL
CSB-EP317991HUL
CSB-BP317991HUL
CSB-MP317991HUL
CSB-EP317991HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translocated intimin receptor Tir (tir), partial CSB-YP317992ENL
CSB-EP317992ENL
CSB-BP317992ENL
CSB-MP317992ENL
CSB-EP317992ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Sucrose-6-phosphate hydrolase (sacA), partial CSB-YP317993ZBM
CSB-EP317993ZBM
CSB-BP317993ZBM
CSB-MP317993ZBM
CSB-EP317993ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Conotoxin Cl6c CSB-YP317994DVU
CSB-EP317994DVU
CSB-BP317994DVU
CSB-MP317994DVU
CSB-EP317994DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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