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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1), partial CSB-YP319003SVG
CSB-EP319003SVG
CSB-BP319003SVG
CSB-MP319003SVG
CSB-EP319003SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein MRP13, mitochondrial (MRP13) CSB-YP319004SVG
CSB-EP319004SVG
CSB-BP319004SVG
CSB-MP319004SVG
CSB-EP319004SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Insulin-2 (ins-b) CSB-YP319005XBE
CSB-EP319005XBE
CSB-BP319005XBE
CSB-MP319005XBE
CSB-EP319005XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 NAD--protein ADP-ribosyltransferase (alt), partial CSB-YP319006EDZ
CSB-EP319006EDZ
CSB-BP319006EDZ
CSB-MP319006EDZ
CSB-EP319006EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6:K15:H31 S-fimbrial protein subunit SfaA (sfaA) CSB-YP319007EGY
CSB-EP319007EGY
CSB-BP319007EGY
CSB-MP319007EGY
CSB-EP319007EGY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 50S ribosomal protein L32e (rpl32e) CSB-YP319008HTJ
CSB-EP319008HTJ
CSB-BP319008HTJ
CSB-MP319008HTJ
CSB-EP319008HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkanal monooxygenase beta chain (luxB) CSB-YP319009PIF
CSB-EP319009PIF
CSB-BP319009PIF
CSB-MP319009PIF
CSB-EP319009PIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA reductase (luxC) CSB-YP319010VDZ
CSB-EP319010VDZ
CSB-BP319010VDZ
CSB-MP319010VDZ
CSB-EP319010VDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyribodipyrimidine photo-lyase (phr) CSB-YP319011SUW
CSB-EP319011SUW
CSB-BP319011SUW
CSB-MP319011SUW
CSB-EP319011SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium fredii Host-inducible protein A CSB-YP319012RKM
CSB-EP319012RKM
CSB-BP319012RKM
CSB-MP319012RKM
CSB-EP319012RKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Megascolia flavifrons Megascoliakinin CSB-YP319013MDH
CSB-EP319013MDH
CSB-BP319013MDH
CSB-MP319013MDH
CSB-EP319013MDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Protamine-1A CSB-YP319014OEI
CSB-EP319014OEI
CSB-BP319014OEI
CSB-MP319014OEI
CSB-EP319014OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial CSB-BP319015HWV
CSB-MP319015HWV
CSB-EP319015HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Tripartite terminase subunit UL28 (UL28), partial CSB-YP319016HJJ
CSB-EP319016HJJ
CSB-BP319016HJJ
CSB-MP319016HJJ
CSB-EP319016HJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Somatotropin-A (gh-a) CSB-YP319017XBE
CSB-EP319017XBE
CSB-BP319017XBE
CSB-MP319017XBE
CSB-EP319017XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic (GAPB) CSB-YP319018FKI
CSB-EP319018FKI
CSB-BP319018FKI
CSB-MP319018FKI
CSB-EP319018FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alpha-factor-transporting ATPase (STE6), partial CSB-YP319019SVG
CSB-EP319019SVG
CSB-BP319019SVG
CSB-MP319019SVG
CSB-EP319019SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boolarra virus Capsid protein alpha (alpha) CSB-YP319020BIJ
CSB-EP319020BIJ
CSB-BP319020BIJ
CSB-MP319020BIJ
CSB-EP319020BIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L29 (rpmC) CSB-YP319021BRJ
CSB-EP319021BRJ
CSB-BP319021BRJ
CSB-MP319021BRJ
CSB-EP319021BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uracil-DNA glycosylase, mitochondrial (UNG1) CSB-YP319022SVG
CSB-EP319022SVG
CSB-BP319022SVG
CSB-MP319022SVG
CSB-EP319022SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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