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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M3 30S ribosomal protein S6 (rpsF) CSB-YP317955SMW
CSB-EP317955SMW
CSB-BP317955SMW
CSB-MP317955SMW
CSB-EP317955SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Ribosome maturation factor RimM (rimM) CSB-YP317956SMW
CSB-EP317956SMW
CSB-BP317956SMW
CSB-MP317956SMW
CSB-EP317956SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Ribonuclease HIII (rnhC) CSB-YP317957SMW
CSB-EP317957SMW
CSB-BP317957SMW
CSB-MP317957SMW
CSB-EP317957SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP317958SMW
CSB-EP317958SMW
CSB-BP317958SMW
CSB-MP317958SMW
CSB-EP317958SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP317959SMV
CSB-EP317959SMV
CSB-BP317959SMV
CSB-MP317959SMV
CSB-EP317959SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP317960SMV
CSB-EP317960SMV
CSB-BP317960SMV
CSB-MP317960SMV
CSB-EP317960SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Segregation and condensation protein A (scpA) CSB-YP317961SMW
CSB-EP317961SMW
CSB-BP317961SMW
CSB-MP317961SMW
CSB-EP317961SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Serine/threonine transporter sstT (sstT), partial CSB-YP317962SMW
CSB-EP317962SMW
CSB-BP317962SMW
CSB-MP317962SMW
CSB-EP317962SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tautomerase SpyM3_0797 (SpyM3_0797) CSB-YP317963SMV
CSB-EP317963SMV
CSB-BP317963SMV
CSB-MP317963SMV
CSB-EP317963SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Formate--tetrahydrofolate ligase 1 (fhs1), partial CSB-YP317964SMW
CSB-EP317964SMW
CSB-BP317964SMW
CSB-MP317964SMW
CSB-EP317964SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA gyrase subunit B (gyrB), partial CSB-YP317965SMW
CSB-EP317965SMW
CSB-BP317965SMW
CSB-MP317965SMW
CSB-EP317965SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Alanine--tRNA ligase (alaS), partial CSB-YP317966SMW
CSB-EP317966SMW
CSB-BP317966SMW
CSB-MP317966SMW
CSB-EP317966SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP317967SMV
CSB-EP317967SMV
CSB-BP317967SMV
CSB-MP317967SMV
CSB-EP317967SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Leucine--tRNA ligase (leuS), partial CSB-YP317968SMW
CSB-EP317968SMW
CSB-BP317968SMW
CSB-MP317968SMW
CSB-EP317968SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP317969SMW
CSB-EP317969SMW
CSB-BP317969SMW
CSB-MP317969SMW
CSB-EP317969SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Tryptophan--tRNA ligase (trpS) CSB-YP317970SMW
CSB-EP317970SMW
CSB-BP317970SMW
CSB-MP317970SMW
CSB-EP317970SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0052 protein SPs1392 (SPs1392) CSB-YP317971SMW
CSB-EP317971SMW
CSB-BP317971SMW
CSB-MP317971SMW
CSB-EP317971SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Putative membrane protein insertion efficiency factor (SPs1589) CSB-YP317972SMW
CSB-EP317972SMW
CSB-BP317972SMW
CSB-MP317972SMW
CSB-EP317972SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0213 protein SPs0786 (SPs0786) CSB-YP317973SMW
CSB-EP317973SMW
CSB-BP317973SMW
CSB-MP317973SMW
CSB-EP317973SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0298 protein SpyM3_0288 (SpyM3_0288) CSB-YP317974SMV
CSB-EP317974SMV
CSB-BP317974SMV
CSB-MP317974SMV
CSB-EP317974SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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