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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rotavirus A Inner capsid protein VP2, partial CSB-YP319409RGJ
CSB-EP319409RGJ
CSB-BP319409RGJ
CSB-MP319409RGJ
CSB-EP319409RGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP319410HKN
CSB-EP319410HKN
CSB-BP319410HKN
CSB-MP319410HKN
CSB-EP319410HKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP319411HKN
CSB-EP319411HKN
CSB-BP319411HKN
CSB-MP319411HKN
CSB-EP319411HKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP319412HKN
CSB-EP319412HKN
CSB-BP319412HKN
CSB-MP319412HKN
CSB-EP319412HKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag) CSB-YP319413HKY
CSB-EP319413HKY
CSB-BP319413HKY
CSB-MP319413HKY
CSB-EP319413HKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Gag polyprotein (gag), partial CSB-YP319414SGX
CSB-EP319414SGX
CSB-BP319414SGX
CSB-MP319414SGX
CSB-EP319414SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.vervet Pol polyprotein (pol) CSB-YP319415SHJ
CSB-EP319415SHJ
CSB-BP319415SHJ
CSB-MP319415SHJ
CSB-EP319415SHJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Virion infectivity factor (vif) CSB-YP319416HLK
CSB-EP319416HLK
CSB-BP319416HLK
CSB-MP319416HLK
CSB-EP319416HLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype U Protein Tat CSB-YP319417HLR
CSB-EP319417HLR
CSB-BP319417HLR
CSB-MP319417HLR
CSB-EP319417HLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Tat (tat) CSB-YP319418SGX
CSB-EP319418SGX
CSB-BP319418SGX
CSB-MP319418SGX
CSB-EP319418SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Vpr (vpr) CSB-YP319419HLK
CSB-EP319419HLK
CSB-BP319419HLK
CSB-MP319419HLK
CSB-EP319419HLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viral myc transforming protein (MYC) CSB-YP319420ATB
CSB-EP319420ATB
CSB-BP319420ATB
CSB-MP319420ATB
CSB-EP319420ATB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional activator AmpR (ampR) CSB-YP319421DTO
CSB-EP319421DTO
CSB-BP319421DTO
CSB-MP319421DTO
CSB-EP319421DTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine adenovirus A serotype 1 Early E1A 11 kDa protein CSB-YP319422MUS
CSB-EP319422MUS
CSB-BP319422MUS
CSB-MP319422MUS
CSB-EP319422MUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus C serotype 5 Peripentonal hexon-associated protein (PIIIA), partial CSB-YP319423HIL
CSB-EP319423HIL
CSB-BP319423HIL
CSB-MP319423HIL
CSB-EP319423HIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chironomus thummi thummi Globin CTT-VIIB-3 (CTT-7B3) CSB-YP319424CIR
CSB-EP319424CIR
CSB-BP319424CIR
CSB-MP319424CIR
CSB-EP319424CIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P4 Transactivation protein (Delta) CSB-YP319425EDH
CSB-EP319425EDH
CSB-BP319425EDH
CSB-MP319425EDH
CSB-EP319425EDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Hemagglutinin-neuraminidase (HN), partial CSB-YP319426NCR1
CSB-EP319426NCR1
CSB-BP319426NCR1
CSB-MP319426NCR1
CSB-EP319426NCR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 3 virus Hemagglutinin-neuraminidase (HN), partial CSB-YP319427HPJ1
CSB-EP319427HPJ1
CSB-BP319427HPJ1
CSB-MP319427HPJ1
CSB-EP319427HPJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Fusion glycoprotein F0 (F), partial CSB-YP319428NCR
CSB-EP319428NCR
CSB-BP319428NCR
CSB-MP319428NCR
CSB-EP319428NCR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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