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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella typhimurium 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP319186SXB
CSB-EP319186SXB
CSB-BP319186SXB
CSB-MP319186SXB
CSB-EP319186SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein D5 (ddpE) CSB-YP319187DKK
CSB-EP319187DKK
CSB-BP319187DKK
CSB-MP319187DKK
CSB-EP319187DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glutenin, high molecular weight subunit DX5 (GLU-1D-1D), partial CSB-YP319188TQN
CSB-BP319188TQN
CSB-MP319188TQN
CSB-EP319188TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viral myc transforming protein (MYC) CSB-YP319189ASR
CSB-EP319189ASR
CSB-BP319189ASR
CSB-MP319189ASR
CSB-EP319189ASR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MLV-related proviral Env polyprotein, partial CSB-YP319190MO
CSB-EP319190MO
CSB-BP319190MO
CSB-MP319190MO
CSB-EP319190MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis Catch-relaxing peptide CSB-YP319191MWU
CSB-EP319191MWU
CSB-BP319191MWU
CSB-MP319191MWU
CSB-EP319191MWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-lactamase 1 (penPC) CSB-YP319192BQJ
CSB-EP319192BQJ
CSB-BP319192BQJ
CSB-MP319192BQJ
CSB-EP319192BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Putative uncharacterized immunity region protein 4 CSB-YP319193BBA
CSB-EP319193BBA
CSB-BP319193BBA
CSB-MP319193BBA
CSB-EP319193BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Putative uncharacterized immunity region protein 8 CSB-YP319194BBA
CSB-EP319194BBA
CSB-BP319194BBA
CSB-MP319194BBA
CSB-EP319194BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Putative uncharacterized immunity region protein 11 CSB-YP319195BBA
CSB-EP319195BBA
CSB-BP319195BBA
CSB-MP319195BBA
CSB-EP319195BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease HII (rnhB) CSB-YP319196ENV
CSB-EP319196ENV
CSB-BP319196ENV
CSB-MP319196ENV
CSB-EP319196ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline sarcoma virus Tyrosine-protein kinase transforming protein Abl (ABL) CSB-YP319197FAY
CSB-EP319197FAY
CSB-BP319197FAY
CSB-MP319197FAY
CSB-EP319197FAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura fuscoviridis Neurotoxin-1 CSB-YP319198ALM
CSB-EP319198ALM
CSB-BP319198ALM
CSB-MP319198ALM
CSB-EP319198ALM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda laticaudata Short neurotoxin B CSB-YP319199LDB
CSB-EP319199LDB
CSB-BP319199LDB
CSB-MP319199LDB
CSB-EP319199LDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Major capsid protein (ORF3) CSB-YP319200PQT
CSB-EP319200PQT
CSB-BP319200PQT
CSB-MP319200PQT
CSB-EP319200PQT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Endoglucanase (eglS) CSB-YP319201BRJ
CSB-BP319201BRJ
CSB-MP319201BRJ
CSB-EP319201BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldocellum saccharolyticum Beta-glucosidase A (bglA) CSB-YP319202CAM
CSB-EP319202CAM
CSB-BP319202CAM
CSB-MP319202CAM
CSB-EP319202CAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-1 restriction enzyme EcoR124II specificity protein (hsdS) CSB-YP319203ENL
CSB-EP319203ENL
CSB-BP319203ENL
CSB-MP319203ENL
CSB-EP319203ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Maltose fermentation regulatory protein MAL63 (MAL63) CSB-YP319204SAC
CSB-EP319204SAC
CSB-BP319204SAC
CSB-MP319204SAC
CSB-EP319204SAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Relaxosome protein TraY (traY) CSB-YP319205ENL
CSB-BP319205ENL
CSB-MP319205ENL
CSB-EP319205ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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