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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nostoc sp. Ferredoxin, heterocyst (fdxH) CSB-YP319960NHR
CSB-EP319960NHR
CSB-BP319960NHR
CSB-MP319960NHR
CSB-EP319960NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelenopsis aperta Mu-agatoxin-Aa1a CSB-YP319961AER
CSB-EP319961AER
CSB-BP319961AER
CSB-MP319961AER
CSB-EP319961AER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine carbamoyltransferase, mitochondrial (argB) CSB-YP319962DOZ
CSB-EP319962DOZ
CSB-BP319962DOZ
CSB-MP319962DOZ
CSB-EP319962DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus terrestris Extracellular globin-3 CSB-YP319963LJN
CSB-EP319963LJN
CSB-BP319963LJN
CSB-MP319963LJN
CSB-EP319963LJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Flagellar filament 41 kDa core protein (fla) CSB-YP319964BUD
CSB-BP319964BUD
CSB-MP319964BUD
CSB-EP319964BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP319965HWQ1
CSB-EP319965HWQ1
CSB-BP319965HWQ1
CSB-MP319965HWQ1
CSB-EP319965HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T5 Probable exonuclease subunit 1 (D12) CSB-YP319966ELS
CSB-EP319966ELS
CSB-BP319966ELS
CSB-MP319966ELS
CSB-EP319966ELS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase (P2), partial CSB-YP319967PUV
CSB-BP319967PUV
CSB-MP319967PUV
CSB-EP319967PUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 Protein P13 (P13) CSB-YP319968PUV
CSB-EP319968PUV
CSB-BP319968PUV
CSB-MP319968PUV
CSB-EP319968PUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP319969IMV
CSB-EP319969IMV
CSB-BP319969IMV
CSB-MP319969IMV
CSB-EP319969IMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Heat shock 70 kDa protein (HSP70), partial CSB-YP319971ZAX
CSB-EP319971ZAX
CSB-BP319971ZAX
CSB-MP319971ZAX
CSB-EP319971ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PTS system lactose-specific EIICB component (lacE), partial CSB-YP319973FKZ
CSB-EP319973FKZ
CSB-BP319973FKZ
CSB-MP319973FKZ
CSB-EP319973FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP319974RFZ
CSB-EP319974RFZ
CSB-BP319974RFZ
CSB-MP319974RFZ
CSB-EP319974RFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine infectious anemia virus Pol polyprotein (pol) CSB-YP319975EFS
CSB-EP319975EFS
CSB-BP319975EFS
CSB-MP319975EFS
CSB-EP319975EFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chandipura virus Nucleoprotein (N) CSB-YP319976CHN
CSB-EP319976CHN
CSB-BP319976CHN
CSB-MP319976CHN
CSB-EP319976CHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Major outer membrane lipoprotein (oprI) CSB-YP319977EZX
CSB-BP319977EZX
CSB-MP319977EZX
CSB-EP319977EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pol polyprotein (pol), partial CSB-YP319978RAM
CSB-EP319978RAM
CSB-BP319978RAM
CSB-MP319978RAM
CSB-EP319978RAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Hemagglutinin-neuraminidase (HN), partial CSB-YP319979MJJ2
CSB-BP319979MJJ2
CSB-MP319979MJJ2
CSB-EP319979MJJ2-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS3411 CSB-YP319980ENL
CSB-EP319980ENL
CSB-BP319980ENL
CSB-MP319980ENL
CSB-EP319980ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline leukemia virus Envelope glycoprotein (env), partial CSB-YP319981FAR
CSB-EP319981FAR
CSB-BP319981FAR
CSB-MP319981FAR
CSB-EP319981FAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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