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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Periplasmic [NiFe] hydrogenase large subunit (hydB), partial CSB-YP320163DJO
CSB-EP320163DJO
CSB-BP320163DJO
CSB-MP320163DJO
CSB-EP320163DJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Dehydrin DHN3 (DHN3) CSB-YP320164HWQ
CSB-EP320164HWQ
CSB-BP320164HWQ
CSB-MP320164HWQ
CSB-EP320164HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Dehydrin DHN2 (DHN2) CSB-YP320165HWQ
CSB-EP320165HWQ
CSB-BP320165HWQ
CSB-MP320165HWQ
CSB-EP320165HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit beta (mcrB) CSB-YP320166MFF
CSB-EP320166MFF
CSB-BP320166MFF
CSB-MP320166MFF
CSB-EP320166MFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase operon protein D (mcrD) CSB-YP320167MFF
CSB-EP320167MFF
CSB-BP320167MFF
CSB-MP320167MFF
CSB-EP320167MFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP320168RHA
CSB-EP320168RHA
CSB-BP320168RHA
CSB-MP320168RHA
CSB-EP320168RHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP320169VEU
CSB-EP320169VEU
CSB-BP320169VEU
CSB-MP320169VEU
CSB-EP320169VEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 8-amino-7-oxononanoate synthase (bioF) CSB-YP320171ENV
CSB-EP320171ENV
CSB-BP320171ENV
CSB-MP320171ENV
CSB-EP320171ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius 50S ribosomal protein L39e (rpl39e) CSB-YP320172FOZ
CSB-EP320172FOZ
CSB-BP320172FOZ
CSB-MP320172FOZ
CSB-EP320172FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grapevine chrome mosaic virus RNA2 polyprotein, partial CSB-YP320173GDF
CSB-EP320173GDF
CSB-BP320173GDF
CSB-MP320173GDF
CSB-EP320173GDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant IgA receptor (arp4) CSB-YP320175SMR
CSB-EP320175SMR
CSB-BP320175SMR
CSB-MP320175SMR
CSB-EP320175SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio baculatus Periplasmic [NiFeSe] hydrogenase large subunit, partial CSB-YP320176DDB
CSB-EP320176DDB
CSB-BP320176DDB
CSB-MP320176DDB
CSB-EP320176DDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L35 (rpmI) CSB-YP320177GFM
CSB-EP320177GFM
CSB-BP320177GFM
CSB-MP320177GFM
CSB-EP320177GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomycin 3'-kinase (str) CSB-YP320178KBG
CSB-EP320178KBG
CSB-BP320178KBG
CSB-MP320178KBG
CSB-EP320178KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wound tumor virus Non-structural protein Pns10 (S9) CSB-YP320180WBC
CSB-EP320180WBC
CSB-BP320180WBC
CSB-MP320180WBC
CSB-EP320180WBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Enhancer of split m8 protein (E (spl)) CSB-YP320181DLU
CSB-EP320181DLU
CSB-BP320181DLU
CSB-MP320181DLU
CSB-EP320181DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chalcone synthase (CHS) CSB-YP320182DOA
CSB-EP320182DOA
CSB-BP320182DOA
CSB-MP320182DOA
CSB-EP320182DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius DNA-binding protein 7e (Saci_0362) CSB-YP320183FOZ
CSB-EP320183FOZ
CSB-BP320183FOZ
CSB-MP320183FOZ
CSB-EP320183FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Listeriolysin O (hly), partial CSB-YP320184LPY
CSB-EP320184LPY
CSB-BP320184LPY
CSB-MP320184LPY
CSB-EP320184LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP320185IFU
CSB-EP320185IFU
CSB-BP320185IFU
CSB-MP320185IFU
CSB-EP320185IFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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