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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Sulfolobus acidocaldarius DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP319306FOZ
CSB-EP319306FOZ
CSB-BP319306FOZ
CSB-MP319306FOZ
CSB-EP319306FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FlmC (flmC) CSB-YP319307ENV
CSB-EP319307ENV
CSB-BP319307ENV
CSB-MP319307ENV
CSB-EP319307ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius 50S ribosomal protein L30e (rpl30e) CSB-YP319308FOZ
CSB-EP319308FOZ
CSB-BP319308FOZ
CSB-MP319308FOZ
CSB-EP319308FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Ecdysone-induced protein 74EF isoform B (Eip74EF), partial CSB-YP319309DLU
CSB-EP319309DLU
CSB-BP319309DLU
CSB-MP319309DLU
CSB-EP319309DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodosporidium toruloides Phenylalanine ammonia-lyase (PAL), partial CSB-YP319310REB
CSB-EP319310REB
CSB-BP319310REB
CSB-MP319310REB
CSB-EP319310REB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis 6-phospho-beta-galactosidase (lacG) CSB-YP319311LNI
CSB-EP319311LNI
CSB-BP319311LNI
CSB-MP319311LNI
CSB-EP319311LNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit beta (mcrB) CSB-YP319312MSD
CSB-EP319312MSD
CSB-BP319312MSD
CSB-MP319312MSD
CSB-EP319312MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Methyl-coenzyme M reductase I operon protein D (mcrD) CSB-YP319313MSQ
CSB-EP319313MSQ
CSB-BP319313MSQ
CSB-MP319313MSQ
CSB-EP319313MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidaminococcus fermentans (R)-2-hydroxyglutaryl-CoA dehydratase subunit beta CSB-YP319314AWA
CSB-EP319314AWA
CSB-BP319314AWA
CSB-MP319314AWA
CSB-EP319314AWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Integrin beta-PS (mys), partial CSB-YP319315DLU
CSB-EP319315DLU
CSB-BP319315DLU
CSB-MP319315DLU
CSB-EP319315DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Female-specific protein transformer (tra) CSB-YP319317DLU
CSB-EP319317DLU
CSB-BP319317DLU
CSB-MP319317DLU
CSB-EP319317DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Photosystem I iron-sulfur center (psaC) CSB-YP319318ZAX
CSB-EP319318ZAX
CSB-BP319318ZAX
CSB-MP319318ZAX
CSB-EP319318ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Suppressor of silencing P0 (ORF0) CSB-YP319319PQV
CSB-EP319319PQV
CSB-BP319319PQV
CSB-MP319319PQV
CSB-EP319319PQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Movement protein (ORF4) CSB-YP319320PQV
CSB-EP319320PQV
CSB-BP319320PQV
CSB-MP319320PQV
CSB-EP319320PQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize chlorotic mottle virus Uncharacterized protein p32 (ORF1) CSB-YP319321MBG
CSB-EP319321MBG
CSB-BP319321MBG
CSB-MP319321MBG
CSB-EP319321MBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize chlorotic mottle virus Capsid protein (ORF4) CSB-YP319322MBG
CSB-EP319322MBG
CSB-BP319322MBG
CSB-MP319322MBG
CSB-EP319322MBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylmalonyl-CoA mutase small subunit (mutA), partial CSB-YP319323PSH
CSB-EP319323PSH
CSB-BP319323PSH
CSB-MP319323PSH
CSB-EP319323PSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Reverse transcriptase-like protein (RTL) CSB-YP319324DSJ
CSB-EP319324DSJ
CSB-BP319324DSJ
CSB-MP319324DSJ
CSB-EP319324DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Immediate-early protein IE180 (IE), partial CSB-YP319325SRH
CSB-EP319325SRH
CSB-BP319325SRH
CSB-MP319325SRH
CSB-EP319325SRH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Light-harvesting protein B800/830/1020 beta-2 chain, partial CSB-YP319326EAQ
CSB-EP319326EAQ
CSB-BP319326EAQ
CSB-MP319326EAQ
CSB-EP319326EAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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