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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum Ras-like protein rasG (rasG) CSB-YP321343DKK
CSB-EP321343DKK
CSB-BP321343DKK
CSB-MP321343DKK
CSB-EP321343DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Foot-and-mouth disease virus Genome polyprotein, partial CSB-YP321344FCG
CSB-EP321344FCG
CSB-BP321344FCG
CSB-MP321344FCG
CSB-EP321344FCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Glutamine synthetase leaf isozyme, chloroplastic CSB-YP321345EUN
CSB-EP321345EUN
CSB-BP321345EUN
CSB-MP321345EUN
CSB-EP321345EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Protocatechuate 3,4-dioxygenase alpha chain (pcaG) CSB-YP321346BXR
CSB-EP321346BXR
CSB-BP321346BXR
CSB-MP321346BXR
CSB-EP321346BXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Early E3B 10.4 kDa protein, partial CSB-YP321347HII
CSB-EP321347HII
CSB-BP321347HII
CSB-MP321347HII
CSB-EP321347HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 35 Early E3 20.6 kDa glycoprotein CSB-YP321348HIH
CSB-EP321348HIH
CSB-BP321348HIH
CSB-MP321348HIH
CSB-EP321348HIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracaudina chilensis Globin-1 CSB-YP321349PBX
CSB-EP321349PBX
CSB-BP321349PBX
CSB-MP321349PBX
CSB-EP321349PBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Aspartate--tRNA ligase, mitochondrial (MSD1), partial CSB-YP321350SVG
CSB-EP321350SVG
CSB-BP321350SVG
CSB-MP321350SVG
CSB-EP321350SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Glutamate--tRNA ligase (gltX) CSB-YP321351RKU
CSB-EP321351RKU
CSB-BP321351RKU
CSB-MP321351RKU
CSB-EP321351RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orthochirus scrobiculosus Toxin Os1 CSB-YP321353OFA
CSB-EP321353OFA
CSB-BP321353OFA
CSB-MP321353OFA
CSB-EP321353OFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Leucoagglutinating phytohemagglutinin (PDLEC2) CSB-YP321354EUN
CSB-EP321354EUN
CSB-BP321354EUN
CSB-MP321354EUN
CSB-EP321354EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosporon cutaneum Phenol hydroxylase, partial CSB-YP321355TIG
CSB-EP321355TIG
CSB-BP321355TIG
CSB-MP321355TIG
CSB-EP321355TIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Pleiotropic regulatory protein (degT) CSB-YP321356GFM
CSB-EP321356GFM
CSB-BP321356GFM
CSB-MP321356GFM
CSB-EP321356GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae AMP deaminase (AMD1), partial CSB-YP321357SVG
CSB-EP321357SVG
CSB-BP321357SVG
CSB-MP321357SVG
CSB-EP321357SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris PII-type proteinase (prt), partial CSB-YP321358LNF
CSB-EP321358LNF
CSB-BP321358LNF
CSB-MP321358LNF
CSB-EP321358LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein papD (papD) CSB-YP321359ENL
CSB-EP321359ENL
CSB-BP321359ENL
CSB-MP321359ENL
CSB-EP321359ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Sperm histone CSB-YP321360CH
CSB-EP321360CH
CSB-BP321360CH
CSB-MP321360CH
CSB-EP321360CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halococcus morrhuae 30S ribosomal protein S12P (rps12p) CSB-YP321361HBP
CSB-EP321361HBP
CSB-BP321361HBP
CSB-MP321361HBP
CSB-EP321361HBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ABC transport system permease protein p69, partial CSB-YP321362MLR
CSB-EP321362MLR
CSB-BP321362MLR
CSB-MP321362MLR
CSB-EP321362MLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Antitoxin PrlF (prlF) CSB-YP321363ENV
CSB-EP321363ENV
CSB-BP321363ENV
CSB-MP321363ENV
CSB-EP321363ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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