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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomyces alboniger Puromycin N-acetyltransferase (pac) CSB-YP319512SNI
CSB-BP319512SNI
CSB-MP319512SNI
CSB-EP319512SNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial protein (fimA) CSB-YP319513DDR
CSB-EP319513DDR
CSB-BP319513DDR
CSB-MP319513DDR
CSB-EP319513DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Choline-phosphate cytidylyltransferase (PCT1) CSB-YP319514SVG
CSB-EP319514SVG
CSB-BP319514SVG
CSB-MP319514SVG
CSB-EP319514SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Sex pheromone cPD1 CSB-YP319515EEF
CSB-EP319515EEF
CSB-BP319515EEF
CSB-MP319515EEF
CSB-EP319515EEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysemys picta bellii Hemoglobin subunit alpha-A (HBAA) CSB-YP319516CKE
CSB-EP319516CKE
CSB-BP319516CKE
CSB-MP319516CKE
CSB-EP319516CKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Apolipophorin-3 CSB-YP319517MPV
CSB-EP319517MPV
CSB-BP319517MPV
CSB-MP319517MPV
CSB-EP319517MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Serine/threonine-protein kinase BGLF4 (BGLF4) CSB-YP319518EFA
CSB-EP319518EFA
CSB-BP319518EFA
CSB-MP319518EFA
CSB-EP319518EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Envelope glycoprotein I (gI), partial CSB-YP319519HJX1
CSB-EP319519HJX1
CSB-BP319519HJX1
CSB-MP319519HJX1
CSB-EP319519HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein UL11 (UL11) CSB-YP319520HJX
CSB-EP319520HJX
CSB-BP319520HJX
CSB-MP319520HJX
CSB-EP319520HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 17.5 kDa protein in tk-vs intergenic region (y06D) CSB-YP319521EDZ
CSB-EP319521EDZ
CSB-BP319521EDZ
CSB-MP319521EDZ
CSB-EP319521EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Endoribonuclease (regB) CSB-YP319522EDZ
CSB-EP319522EDZ
CSB-BP319522EDZ
CSB-MP319522EDZ
CSB-EP319522EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 25.0 kDa protein in regB-denV intergenic region (y06K) CSB-YP319523EDZ
CSB-EP319523EDZ
CSB-BP319523EDZ
CSB-MP319523EDZ
CSB-EP319523EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 6.6 kDa protein in segB-ipI intergenic region (y07D) CSB-YP319524EDZ
CSB-EP319524EDZ
CSB-BP319524EDZ
CSB-MP319524EDZ
CSB-EP319524EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 7.9 kDa protein in mobB-Gp55 intergenic region (y03G) CSB-YP319525EDZ
CSB-EP319525EDZ
CSB-BP319525EDZ
CSB-MP319525EDZ
CSB-EP319525EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Probable mobile endonuclease B (mobB) CSB-YP319526EDZ
CSB-EP319526EDZ
CSB-BP319526EDZ
CSB-MP319526EDZ
CSB-EP319526EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail sheath protein Gp18 (18), partial CSB-YP319527EDZ
CSB-EP319527EDZ
CSB-BP319527EDZ
CSB-MP319527EDZ
CSB-EP319527EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp26 (26) CSB-YP319528EDZ
CSB-EP319528EDZ
CSB-BP319528EDZ
CSB-MP319528EDZ
CSB-EP319528EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 RNA polymerase-associated protein Gp33 (33) CSB-YP319529EDZ
CSB-EP319529EDZ
CSB-BP319529EDZ
CSB-MP319529EDZ
CSB-EP319529EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Protein Gp59 (59) CSB-YP319530EDZ
CSB-EP319530EDZ
CSB-BP319530EDZ
CSB-MP319530EDZ
CSB-EP319530EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella protothecoides Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP319531CJG1
CSB-EP319531CJG1
CSB-BP319531CJG1
CSB-MP319531CJG1
CSB-EP319531CJG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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