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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant L-lactate dehydrogenase (ldh) CSB-YP319920BAJ
CSB-EP319920BAJ
CSB-BP319920BAJ
CSB-MP319920BAJ
CSB-EP319920BAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein MRP2, mitochondrial (MRP2) CSB-YP319921SVG
CSB-EP319921SVG
CSB-BP319921SVG
CSB-MP319921SVG
CSB-EP319921SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Fasciclin-1 (Fas1), partial CSB-YP319922DLU
CSB-EP319922DLU
CSB-BP319922DLU
CSB-MP319922DLU
CSB-EP319922DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 7, chloroplastic (CAB7), partial CSB-YP319923SZY1
CSB-EP319923SZY1
CSB-BP319923SZY1
CSB-MP319923SZY1
CSB-EP319923SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reaction center protein M chain (pufM), partial CSB-YP319924RMA1
CSB-EP319924RMA1
CSB-BP319924RMA1
CSB-MP319924RMA1
CSB-EP319924RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Alanine racemase (alr) CSB-YP319925GFM
CSB-EP319925GFM
CSB-BP319925GFM
CSB-MP319925GFM
CSB-EP319925GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Anti-sigma F factor (spoIIAB) CSB-YP319926BRJ
CSB-EP319926BRJ
CSB-BP319926BRJ
CSB-MP319926BRJ
CSB-EP319926BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Severin (sevA) CSB-YP319927DKK
CSB-EP319927DKK
CSB-BP319927DKK
CSB-MP319927DKK
CSB-EP319927DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dolichovespula maculata Venom allergen 5.01 CSB-YP319928DEW
CSB-EP319928DEW
CSB-BP319928DEW
CSB-MP319928DEW
CSB-EP319928DEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant rRNA adenine N-6-methyltransferase (ermBC) CSB-YP319929ENL
CSB-EP319929ENL
CSB-BP319929ENL
CSB-MP319929ENL
CSB-EP319929ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic CSB-YP319930SZY
CSB-EP319930SZY
CSB-BP319930SZY
CSB-MP319930SZY
CSB-EP319930SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mating pheromone Er-1/Er-3 (MAT1) CSB-YP319931EIL
CSB-EP319931EIL
CSB-BP319931EIL
CSB-MP319931EIL
CSB-EP319931EIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triturus cristatus Hemoglobin subunit beta-2 (HBB2) CSB-YP319932TIS
CSB-EP319932TIS
CSB-BP319932TIS
CSB-MP319932TIS
CSB-EP319932TIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S11P (rps11p) CSB-YP319933HTJ
CSB-EP319933HTJ
CSB-BP319933HTJ
CSB-MP319933HTJ
CSB-EP319933HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribulose bisphosphate carboxylase small chain 1A, chloroplastic (RBCS-1A) CSB-YP319934DOA
CSB-EP319934DOA
CSB-BP319934DOA
CSB-MP319934DOA
CSB-EP319934DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Wide host range virA protein (virA), partial CSB-YP319935AEZ
CSB-EP319935AEZ
CSB-BP319935AEZ
CSB-MP319935AEZ
CSB-EP319935AEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein PET54 (PET54) CSB-YP319936SVG
CSB-EP319936SVG
CSB-BP319936SVG
CSB-MP319936SVG
CSB-EP319936SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mating-type P-specific polypeptide Pc (matPc) CSB-YP319937SXU
CSB-EP319937SXU
CSB-BP319937SXU
CSB-MP319937SXU
CSB-EP319937SXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18) CSB-YP319938SVG
CSB-EP319938SVG
CSB-BP319938SVG
CSB-MP319938SVG
CSB-EP319938SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Fusion glycoprotein F0 (F), partial CSB-YP319939NCZ
CSB-EP319939NCZ
CSB-BP319939NCZ
CSB-MP319939NCZ
CSB-EP319939NCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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